Identification of pathways, gene networks, and paralogous gene families in Daphnia pulex responding to exposure to the toxic cyanobacterium Microcystis aeruginosa.
Identification of pathways, gene networks, and paralogous gene families in Daphnia pulex responding to exposure to the toxic cyanobacterium Microcystis aeruginosa.
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DOI：
10.1021/es301100j
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发表时间：
2012-08-07
影响因子：
11.4
通讯作者：
De Schamphelaere, Karel A. C.
中科院分区：
文献类型：
--
作者：
Asselman, Jana;De Coninck, Dieter I. M.;Glaholt, Stephen;Colbourne, John K.;Janssen, Cohn R.;Shaw, Joseph R.;De Schamphelaere, Karel A. C.
虽然蓝细菌产生广泛的天然毒素，影响水生生物，食物网和水质，毒性机制仍然没有得到充分的了解。在这里，我们实施了一个全基因组表达微阵列，以确定途径，基因网络和旁系同源基因家族响应微囊藻应力蚤状蚤。因此，一个敏感的分离的新生儿给予微囊藻污染的饮食，以对比与对照饮食16天。微阵列揭示了2247个响应微囊藻的差异表达（DE）基因（占阵列的7.6%），其中17%是谱系特异性的（即，这些基因与目前可用数据库中的任何其它基因没有可检测的同源性），并且49%是基因重复（旁系同源物）。我们确定了四个途径/基因网络和八个旁系同源基因家族受微囊藻影响。核糖体的差异调节，包括3个旁系同源基因家族编码的40 S，60 S和线粒体核糖体蛋白，表明微囊藻对蛋白质合成的影响。蚤。此外，氧化磷酸化途径（包括NADH亚醌氧化还原酶基因家族）和胰蛋白酶旁系同源基因家族（D. pulex）可以解释为什么基于能量预算考虑，适合度降低。
Although cyanobacteria produce a wide range of natural toxins that impact aquatic organisms, food webs and water quality, the mechanisms of toxicity are still insufficiently understood. Here, we implemented a whole-genome expression microarray to identify pathways, gene networks and paralogous gene families responsive to Microcystis stress in Daphnia pulex. Therefore, neonates of a sensitive isolate were given a diet contaminated with Microcystis to contrast with those given a control diet for sixteen days. The microarray revealed 2247 differentially expressed (DE) genes (7.6% of the array) in response to Microcystis, of which 17% are lineage specific( i.e., these genes have no detectable homology to any other gene in currently available databases) and 49% are gene duplicates (paralogs). We identified four pathways/gene networks and eight paralogous gene families affected by Microcystis. Differential regulation of the ribosome, including 3 paralogous gene families encoding 40S, 60S and mitochondrial ribosomal proteins, suggests an impact of Microcystis on protein synthesis of D. pulex. In addition, differential regulation of the oxidative phosphorylation pathway (including the NADH ubquinone oxidoreductase gene family) and the trypsin paralogous gene family (a major component of the digestive system in D. pulex) could explain why fitness is reduced based on energy budget considerations.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
--
作者：
Fisher, RA
通讯作者：
Fisher, RA
影响因子：
2.1
作者：
Ishida, K;Okita, Y;Murakami, M
通讯作者：
Murakami, M
影响因子：
8.3
作者：
Ding, WX;Shen, HM;Ong, CN
通讯作者：
Ong, CN
DOI：
10.1126/science.1197761
发表时间：
2011-02-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colbourne JK;Pfrender ME;Gilbert D;Thomas WK;Tucker A;Oakley TH;Tokishita S;Aerts A;Arnold GJ;Basu MK;Bauer DJ;Cáceres CE;Carmel L;Casola C;Choi JH;Detter JC;Dong Q;Dusheyko S;Eads BD;Fröhlich T;Geiler-Samerotte KA;Gerlach D;Hatcher P;Jogdeo S;Krijgsveld J;Kriventseva EV;Kültz D;Laforsch C;Lindquist E;Lopez J;Manak JR;Muller J;Pangilinan J;Patwardhan RP;Pitluck S;Pritham EJ;Rechtsteiner A;Rho M;Rogozin IB;Sakarya O;Salamov A;Schaack S;Shapiro H;Shiga Y;Skalitzky C;Smith Z;Souvorov A;Sung W;Tang Z;Tsuchiya D;Tu H;Vos H;Wang M;Wolf YI;Yamagata H;Yamada T;Ye Y;Shaw JR;Andrews J;Crease TJ;Tang H;Lucas SM;Robertson HM;Bork P;Koonin EV;Zdobnov EM;Grigoriev IV;Lynch M;Boore JL
通讯作者：
Boore JL