Spermidine promotes mating and fertilization efficiency in model organisms.
Spermidine promotes mating and fertilization efficiency in model organisms.
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DOI：
10.4161/cc.23199
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发表时间：
2013-01-15
期刊：
影响因子：
--
通讯作者：
Madeo F
中科院分区：
文献类型：
--
作者：
Bauer MA;Carmona-Gutiérrez D;Ruckenstuhl C;Reisenbichler A;Megalou EV;Eisenberg T;Magnes C;Jungwirth H;Sinner FM;Pieber TR;Fröhlich KU;Kroemer G;Tavernarakis N;Madeo F
亚精胺是一种天然存在的多胺，参与多种生物过程，包括 DNA 代谢、自噬和衰老。与其他多胺一样，亚精胺对于多个阶段的成功繁殖也是不可或缺的。然而，对实际受精过程（即卵母细胞与精母细胞的融合）的直接影响仍然不确定。为了探索这种可能性，我们建立了酿酒酵母的交配过程作为高等真核生物受精的模型。在人类受精过程中，精子获能，顶体反应对于穿透卵母细胞是必要的。同样，性活跃的酵母会形成一种称为“shmoo”的突起，作为交配的先决条件。在这项研究中，我们证明信息素诱导的 shmoo 形成需要亚精胺。此外，我们还发现亚精胺对于酵母的交配以及线虫的卵子受精至关重要。在这两种情况下，这都独立于自噬而发生。在合成过程中，我们确定亚精胺是单细胞和多细胞模型生物体中的重要交配成分，支持受精相关细胞融合机制的前所未有的进化保守性。
Spermidine is a naturally occurring polyamine involved in multiple biological processes, including DNA metabolism, autophagy and aging. Like other polyamines, spermidine is also indispensable for successful reproduction at several stages. However, a direct influence on the actual fertilization process, i.e., the fusion of an oocyte with a spermatocyte, remains uncertain. To explore this possibility, we established the mating process in the yeast Saccharomyces cerevisiae as a model for fertilization in higher eukaryotes. During human fertilization, the sperm capacitates and the acrosome reaction is necessary for penetration of the oocyte. Similarly, sexually active yeasts form a protrusion called “shmoo” as a prerequisite for mating. In this study, we demonstrate that pheromone-induced shmoo formation requires spermidine. In addition, we show that spermidine is essential for mating in yeast as well as for egg fertilization in the nematode Caenorhabditis elegans. In both cases, this occurs independently from autophagy. In synthesis, we identify spermidine as an important mating component in unicellular and multicellular model organisms, supporting an unprecedented evolutionary conservation of the mechanisms governing fertilization-related cellular fusion.
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发表时间：
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期刊：
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影响因子：
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