Genome-Wide Analysis, Evolutionary History and Response of ALMT Family to Phosphate Starvation in Brassica napus.
Genome-Wide Analysis, Evolutionary History and Response of ALMT Family to Phosphate Starvation in Brassica napus.
复制标题
甘蓝型油菜ALMT家族的全基因组分析、进化历史及对磷饥饿的响应
DOI：
10.3390/ijms22094625
复制
发表时间：
2021-04-28
影响因子：
5.6
通讯作者：
Shi L
中科院分区：
文献类型：
--
作者：
Din I;Ullah I;Wang W;Zhang H;Shi L
磷的有效性低是植物生长的主要限制因素之一，特别是在酸性土壤中。一个可能的机制，以提高利用稀疏可溶性磷形式是苹果酸的分泌在植物中的铝激活的苹果酸转运蛋白（ALMT）基因家族。尽管ALMT基因家族在植物生物学中具有重要意义，但在油菜（Brassica napus; B. napus），一种异源四倍体作物，被揭开。在此，我们对B中的ALMT进行了全基因组鉴定和表征。油菜，确定其在不同组织中的基因表达和监测BnaALMTs在根和叶中的转录调控在充足和缺乏磷供应。共鉴定出39个BnaALMT基因，并根据蛋白质序列将其聚类为5个分支。共线性分析表明，在B区，大部分BnaALMT基因在BnaALMT成员间具有共线性关系。napus的基因组序列分析表明，BnaALMTs的进化除了基因片段的重复外，还存在着全基因组的重复（多倍体）。RNA-seq分析表明，大多数BnaALMT基因在根和叶组织中优先表达。其中，BnaC 08 g13520 D、BnaC 08 g15170 D、BnaC 08 g15180 D、BnaC 08 g13490 D、BnaC 08 g13500 D、BnaA 08 g26960 D、BnaC 05 g14120 D、BnaA 06 g12560 D、BnaC 05 g20630 D、BnaA 07 g 02630 D、BnaA 04 g15700 D在B中表达显著上调。缺磷条件下油菜根系和叶片的生长受到抑制。本研究分析了ALMT家族在B中的进化和表达。这将有助于进一步研究它们通过分泌有机酸提高土壤磷素有效性的作用。
Low phosphorus (P) availability is one of the major constraints to plant growth, particularly in acidic soils. A possible mechanism for enhancing the use of sparsely soluble P forms is the secretion of malate in plants by the aluminum-activated malate transporter (ALMT) gene family. Despite its significance in plant biology, the identification of the ALMT gene family in oilseed rape (Brassica napus; B. napus), an allotetraploid crop, is unveiled. Herein, we performed genome-wide identification and characterization of ALMTs in B. napus, determined their gene expression in different tissues and monitored transcriptional regulation of BnaALMTs in the roots and leaves at both a sufficient and a deficient P supply. Thirty-nine BnaALMT genes were identified and were clustered into five branches in the phylogenetic tree based on protein sequences. Collinearity analysis revealed that most of the BnaALMT genes shared syntenic relationships among BnaALMT members in B. napus, which suggested that whole-genome duplication (polyploidy) played a major driving force for BnaALMTs evolution in addition to segmental duplication. RNA-seq analyses showed that most BnaALMT genes were preferentially expressed in root and leaf tissues. Among them, the expression of BnaC08g13520D, BnaC08g15170D, BnaC08g15180D, BnaC08g13490D, BnaC08g13500D, BnaA08g26960D, BnaC05g14120D, BnaA06g12560D, BnaC05g20630D, BnaA07g02630D, BnaA04g15700D were significantly up-regulated in B. napus roots and leaf at a P deficient supply. The current study analyzes the evolution and the expression of the ALMT family in B. napus, which will help in further research on their role in the enhancement of soil P availability by secretion of organic acids.
登录
查看更多内容
影响因子：
27.5
作者：
Liu, Fang;Chang, Xiao-Jian;Lian, Xing-Ming
通讯作者：
Lian, Xing-Ming
影响因子：
7.2
作者：
Furuichi, Takuya;Sasaki, Takayuki;Yamamoto, Yoko
通讯作者：
Yamamoto, Yoko
影响因子：
7.4
作者：
Liao, Hong;Wan, Huiyan;Kochian, Leon V.
通讯作者：
Kochian, Leon V.
影响因子：
6.7
作者：
Sukweenadhi, Johan;Kim, Yeon-Ju;Yang, Deok Chun
通讯作者：
Yang, Deok Chun
大豆 GmALMT 家族基因的特征以及 GmALMT5 响应磷酸盐饥饿的功能
影响因子：
11.4
作者：
Peng, Wenting;Wu, Weiwei;Liao, Hong
通讯作者：
Liao, Hong