Accelerated senescence of endothelial progenitor cells in hypertension is related to the reduction of calcitonin gene-related peptide
Accelerated senescence of endothelial progenitor cells in hypertension is related to the reduction of calcitonin gene-related peptide
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高血压内皮祖细胞加速衰老与降钙素基因相关肽减少有关
DOI：
10.1097/hjh.0b013e3283399326
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发表时间：
2010-05
影响因子：
4.9
通讯作者：
Jun Peng
中科院分区：
文献类型：
--
作者：
Chang-Ping Hu;Xiao-Ping Chen;Zhi Zhou;Ting-Ting Li;Dai li;Chen-Jing Wang;Yuan-Jian Li;Jun Peng
目的探讨高血压时内皮祖细胞（EPCs）的加速衰老是否与降钙素基因相关肽（CGRP）的减少有关。方法和结果在体内研究中，血浆CGRP水平和衰老的EPCs的数量在高血压的人和动物，从中分离的EPCs检查CGRP的生产。此外，吴茱萸次碱，作为一种代理或工具，以刺激CGRP的生产，在高血压动物。在体外研究吴茱萸次碱对血管紧张素II诱导的EPCs衰老的影响。结果表明，高血压病患者外周血循环中衰老EPCs数量显著增加，血浆CGRP水平降低，EPCs中CGRP mRNA表达减少。在自发性高血压大鼠中，给予吴茱萸次碱逆转EPC衰老，沿着CGRP产生的升高。在血管紧张素II诱导的EPCs衰老过程中，CGRP mRNA表达降低，吴茱萸次碱可逆转这一趋势。吴茱萸次碱对EPCs的作用在瞬时受体电位香草酸1的选择性拮抗剂辣椒平的存在下被取消。结论CGRP可能作为内源性保护物质对抗高血压时EPCs的衰老，高血压时EPCs的加速衰老与CGRP的减少有关。
Objectives To explore whether the accelerated senescence of endothelial progenitor cells (EPCs) is related to the reduction of calcitonin gene-related peptide (CGRP) in hypertension. Methods and results In-vivo studies, plasma levels of CGRP and the number of senescent EPCs were measured in hypertensive humans and animals, from which the EPCs were isolated to examine the production of CGRP. Moreover, rutaecarpine, as an agent or tool to stimulate CGRP production, was used in hypertensive animals. The effects of rutaecarpine on angiotensin II-induced EPCs senescence were evaluated in vitro. The results showed that the number of circulating senescent EPCs was significantly increased in hypertension concomitantly with the decreased plasma level of CGRP and the decreased CGRP mRNA expression in EPCs. Administration of rutaecarpine reversed EPC senescence along with an elevation in CGRP production in spontaneously hypertensive rats. In the angiotensin II-induced EPCs senescence, the CGRP mRNA expression was reduced, which was reversed by rutaecarpine. The effect of rutaecarpine on EPCs was canceled in the presence of capsazepine, a selective antagonist of transient receptor potential vanilloid 1. Conclusion The results suggest that CGRP may work as an endogenous protective substance to counteract EPCs senescence in hypertension and the accelerated EPCs senescence in hypertension was related to the reduction of CGRP.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
20.3
作者：
S. Wimalawansa
通讯作者：
S. Wimalawansa
瞬时受体电位香草酸1介导的内皮细胞源性降钙素基因相关肽的表达和分泌
影响因子：
--
作者：
Luo, Dan;Zhang, Yi-Wei;Li, Yuan-Jian
通讯作者：
Li, Yuan-Jian
影响因子：
4.9
作者：
Jianping Li;Huawei Zhao;S. Supowit;D. DiPette;Donna H. Wang
通讯作者：
Jianping Li;Huawei Zhao;S. Supowit;D. DiPette;Donna H. Wang
影响因子：
4
作者：
Benndorf, Ralf A.;Gehling, Ursula M.;Boeger, Rainer
通讯作者：
Boeger, Rainer