3'-Daidzein sulfonate sodium protects against memory impairment and hippocampal damage caused by chronic cerebral hypoperfusion.
3'-Daidzein sulfonate sodium protects against memory impairment and hippocampal damage caused by chronic cerebral hypoperfusion.
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3α-大豆苷元磺酸钠可预防慢性脑灌注不足引起的记忆障碍和海马损伤
DOI：
10.4103/1673-5374.237119
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发表时间：
2018-09
影响因子：
6.1
通讯作者：
Xiao H
中科院分区：
文献类型：
--
作者：
Li X;Liu RZ;Zeng Q;Huang ZH;Zhang JD;Liu ZL;Zeng J;Xiao H
3‘-大豆苷元磺酸钠(DSS)是以大豆苷元为原料合成的一种新型水溶性化合物。大豆苷元是一种具有神经生物学调节作用的大豆异黄酮。在这项研究中，我们假设DSS在神经生物学中的调节作用对海马区损伤和记忆障碍具有治疗作用。为了验证这一假说，我们采用双血管结扎法建立了颈总动脉永久性闭塞的慢性脑低灌注(CCH)大鼠模型。造模3周后灌胃给予DSS 0.1、0.2、0.4 mg/kg，1次/d，连续5周。采用Morris水迷宫实验观察CCH对小鼠学习记忆的影响。TUNEL法检测大鼠海马区CA1、CA3区及齿状回细胞凋亡情况。苏木精-伊红染色观察大鼠海马CA1、CA3区和齿状回神经元的形态。Western印迹法检测海马PKA/ERK1/2/CREB信号通路中PKA、ERK1/2和CREB的磷酸化。结果表明，DSS治疗能显著改善CCH大鼠的学习记忆障碍，减少海马区CA1、CA3区和齿状回神经元的凋亡，增加海马区PKA、ERK1/2和CREB的磷酸化。提示DSS可能通过激活PKA/ERK1/2/CREB信号通路，对CCH所致的记忆损伤和海马神经元损伤具有保护作用。
3′-Daidzein sulfonate sodium (DSS) is a new synthetic water-soluble compound derived from daidzein, a soya isoflavone that plays regulatory roles in neurobiology. In this study, we hypothesized that the regulatory role of DSS in neurobiology exhibits therapeutic effects on hippocampal damage and memory impairment. To validate this hypothesis, we established rat models of chronic cerebral hypoperfusion (CCH) by the permanent occlusion of the common carotid arteries using the two-vessel occlusion method. Three weeks after modeling, rat models were intragastrically administered 0.1, 0.2, and 0.4 mg/kg DSS, once a day, for 5 successive weeks. The Morris water maze test was performed to investigate CCH-induced learning and memory deficits. TUNEL assay was used to analyze apoptosis in the hippocampal CA1, CA3 regions and dentate gyrus. Hematoxylin-eosin staining was performed to observe the morphology of neurons in the hippocampal CA1, CA3 regions and dentate gyrus. Western blot analysis was performed to investigate the phosphorylation of PKA, ERK1/2 and CREB in the hippocampal PKA/ERK1/2/CREB signaling pathway. Results showed that DSS treatment greatly improved the learning and memory deficits of rats with CCH, reduced apoptosis of neurons in the hippocampal CA1, CA3 regions and dentate gyrus, and increased the phosphorylation of PKA, ERK1/2, and CREB in the hippocampus. These findings suggest that DSS protects against CCH-induced memory impairment and hippocampal damage possibly through activating the PKA/ERK1/2/CREB signaling pathway.
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影响因子：
6.1
作者：
Yuan W;Chen Q;Zeng J;Xiao H;Huang ZH;Li X;Lei Q
通讯作者：
Lei Q
影响因子：
2.9
作者：
Farkas, E;Institóris, A;Bari, F
通讯作者：
Bari, F
影响因子：
3.6
作者：
Thorp, Alicia A.;Sinn, Natalie;Howe, Peter R. C.
通讯作者：
Howe, Peter R. C.
肌肽对慢性脑灌注不足引起的白质损伤的保护作用。
影响因子：
6.1
作者：
Ma J;Bo SH;Lu XT;Xu AJ;Zhang J
通讯作者：
Zhang J
影响因子：
4.8
作者：
Lana D;Melani A;Pugliese AM;Cipriani S;Nosi D;Pedata F;Giovannini MG
通讯作者：
Giovannini MG