Increased bisecting N-acetylglucosamine and decreased branched chain glycans of N-linked glycoproteins in expressed prostatic secretions associated with prostate cancer progression.
Increased bisecting N-acetylglucosamine and decreased branched chain glycans of N-linked glycoproteins in expressed prostatic secretions associated with prostate cancer progression.
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DOI：
10.1002/prca.201200134
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Drake RR
中科院分区：
文献类型：
--
作者：
Nyalwidhe JO;Betesh LR;Powers TW;Jones EE;White KY;Burch TC;Brooks J;Watson MT;Lance RS;Troyer DA;Semmes OJ;Mehta A;Drake RR
利用富含糖蛋白（如前列腺特异性抗原（PSA）和前列腺酸性磷酸酶（PAP））的前列腺液，本研究的目的是确定与前列腺癌状态相关的聚糖的结构类型和相对丰度，以便随后用于新兴的基于质谱的糖肽分析平台。通过MALDI-TOF分析、正相高效液相色谱分离和三重四极柱质谱分析三种互补方法对PAP糖肽进行n -链聚糖分析，收集反映前列腺癌不同阶段的表达前列腺分泌物（EPS）和外泌体样本。来自不同EPS液体的n -链聚糖分析表明，较大的支链三和四天线聚糖在全球范围内减少。不同的外糖苷酶治疗表明，与疾病严重程度相关的n -乙酰氨基葡萄糖的分割显著增加。对侵袭性前列腺癌池中PAP的n链糖肽位点进行三重四极杆质谱分析，与聚糖谱分析数据交叉参考。EPS液中糖基化的变化反映了前列腺癌的临床状态。在单个样本的糖肽水平上定义这些分子特征可以改善当前的诊断和预后方法。
Using prostatic fluids rich in glycoproteins like prostate specific antigen (PSA) and prostatic acid phosphatase (PAP) , the goal of this study was to identify the structural types and relative abundance of glycans associated with prostate cancer status for subsequent use in emerging mass spectrometry-based glycopeptide analysis platforms. A series of pooled samples of expressed prostatic secretions (EPS) and exosomes reflecting different stages of prostate cancer disease were used for N-linked glycan profiling by three complementary methods, MALDI-TOF profiling, normal-phase HPLC separation, and triple quadropole MS analysis of PAP glycopeptides. Glycan profiling of N-linked glycans from different EPS fluids indicated a global decrease in larger branched tri- and tetra-antennary glycans. Differential exoglycosidase treatments indicated a substantial increase in bisecting N-acetylglucosamines correlated with disease severity. A triple quadrupole MS analysis of the N-linked glycopeptides sites from PAP in aggressive prostate cancer pools was done to cross-reference with the glycan profiling data. Changes in glycosylation as detected in EPS fluids reflect the clinical status of prostate cancer. Defining these molecular signatures at the glycopeptide level in individual samples could improve current approaches of diagnosis and prognosis.
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影响因子：
3.3
作者：
Drake, Richard R.;White, Krista Y.;Fuller, Thomas W.;Igwe, Elena;Clements, Mary Ann;Nyalwidhe, Julius O.;Given, Robert W.;Lance, Raymond S.;Semmes, O. John
通讯作者：
Semmes, O. John
影响因子：
7.4
作者：
Marleau AM;Chen CS;Joyce JA;Tullis RH
通讯作者：
Tullis RH
影响因子：
2.6
作者：
Iczkowski, Kenneth A;Lucia, M Scott
通讯作者：
Lucia, M Scott
影响因子：
3.9
作者：
Arienti, G;Carlini, E;Palmerini, CA
通讯作者：
Palmerini, CA
影响因子：
19.6
作者：
Alvarez, M. Lucrecia;Khosroheidari, Mahdieh;DiStefano, Johanna K.
通讯作者：
DiStefano, Johanna K.