Synthesis and Cytotoxicity of Y(2)O(3) Nanoparticles of Various Morphologies.
Synthesis and Cytotoxicity of Y(2)O(3) Nanoparticles of Various Morphologies.
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DOI：
10.1007/s11671-009-9445-0
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发表时间：
2009-11-24
影响因子：
--
通讯作者：
Riman, Richard E.
中科院分区：
文献类型：
--
作者：
Andelman, Tamar;Gordonov, Simon;Busto, Gabrielle;Moghe, Prabhas V.;Riman, Richard E.
随着纳米技术领域的不断发展，评价纳米颗粒的细胞毒性对其在生物医学领域的进一步应用具有重要意义。在这里，我们报道了不同形态氧化钇纳米颗粒的合成、表征和细胞毒性，氧化钇是一种很有前途的生物成像材料。通过溶剂热法和水热法合成了球形、棒状和血小板状纳米颗粒，并通过透射电子显微镜（TEM）、x射线衍射（XRD）、光散射、表面积分析、热重分析（TGA）和zeta电位测量对其进行了表征。然后对纳米颗粒对人包皮成纤维细胞（HFF）的细胞毒性进行了测试，目的是阐明纳米颗粒的特性对细胞毒性的影响。不同形态的细胞反应不同，球形颗粒对HFF细胞无细胞毒性，棒状颗粒促进细胞增殖，血小板颗粒具有明显的细胞毒性。然而，由于通过表征技术确定的纳米颗粒化学性质的差异，很难将细胞毒性反应归因于颗粒形态。相反，血小板样品的细胞毒性是由于稳定配体，油胺，它在该样品中存在较高水平。这项研究证明了纳米颗粒化学对体外细胞毒性的重要性，并强调了全面表征纳米颗粒的重要性，这是理解纳米颗粒细胞毒性的先决条件。
As the field of nanotechnology continues to grow, evaluating the cytotoxicity of nanoparticles is important in furthering their application within biomedicine. Here, we report the synthesis, characterization, and cytotoxicity of nanoparticles of different morphologies of yttrium oxide, a promising material for biological imaging applications. Nanoparticles of spherical, rod-like, and platelet morphologies were synthesized via solvothermal and hydrothermal methods and characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), light scattering, surface area analysis, thermogravimetric analysis (TGA), and zeta potential measurements. Nanoparticles were then tested for cytotoxicity with human foreskin fibroblast (HFF) cells, with the goal of elucidating nanoparticle characteristics that influence cytotoxicity. Cellular response was different for the different morphologies, with spherical particles exhibiting no cytotoxicity to HFF cells, rod-like particles increasing cell proliferation, and platelet particles markedly cytotoxic. However, due to differences in the nanoparticle chemistry as determined through the characterization techniques, it is difficult to attribute the cytotoxicity responses to the particle morphology. Rather, the cytotoxicity of the platelet sample appears due to the stabilizing ligand, oleylamine, which was present at higher levels in this sample. This study demonstrates the importance of nanoparticle chemistry on in vitro cytotoxicity, and highlights the general importance of thorough nanoparticle characterization as a prerequisite to understanding nanoparticle cytotoxicity.
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影响因子：
11.4
作者：
Jia, G;Wang, HF;Guo, XB
通讯作者：
Guo, XB
DOI：
10.1073/pnas.152463399
发表时间：
2002-10-01
影响因子：
11.1
作者：
Åkerman, ME;Chan, WCW;Ruoslahti, E
通讯作者：
Ruoslahti, E
影响因子：
2.7
作者：
GIESCHE, H;MATIJEVIC, E
通讯作者：
MATIJEVIC, E
影响因子：
2.1
作者：
De, GJ;Qin, WP;Cui, Y
通讯作者：
Cui, Y
影响因子：
6.1
作者：
Bhatia, Sujata K.;Yetter, Ann B.
通讯作者：
Yetter, Ann B.