Microbial Community Transplant Results in Increased and Long-Term Oxalate Degradation.
Microbial Community Transplant Results in Increased and Long-Term Oxalate Degradation.
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微生物群落移植会导致草酸盐降解和长期降解。
DOI：
10.1007/s00248-016-0800-2
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发表时间：
2016-08
影响因子：
3.6
通讯作者：
Dearing, M. Denise
中科院分区：
文献类型：
--
作者：
Miller, Aaron W.;Oakeson, Kelly F.;Dale, Colin;Dearing, M. Denise
肠道微生物对膳食草酸盐的降解至关重要，这种功能可能在降低肾结石的发病率方面发挥作用。然而，许多草酸盐降解细菌对抗生素敏感，并且使用草酸盐降解益生菌仅导致尿草酸盐的短暂减少。本研究的目的是确定使用整个社区的微生物移植从野生哺乳动物草食动物，Neotoma albigula，以增加草酸盐降解在实验室大鼠，褐家鼠长期的有效性。我们量化了实验室大鼠在微生物移植后立即以及在微生物移植后2个月和9个月的时间间隔内总草酸盐降解的变化。此外，我们使用16S rRNA基因高变区的高通量Illumina测序跟踪了实验室大鼠的粪便微生物群，无论是否进行微生物移植。微生物移植导致草酸盐降解显著增加，这种效果在最初移植后持续9个月。功能的持久性得到了证实的转移，和持久性的一组细菌以前与草酸消耗在N。albigula，包括一种来自草酸盐属的厌氧细菌，以其使用草酸盐作为唯一碳源的能力而闻名。这项研究的结果表明，全群落微生物移植是一种有效的手段，持久的殖民化的琥珀酸降解细菌在哺乳动物肠道。
Gut microbes are essential for the degradation of dietary oxalate, and this function may play a role in decreasing the incidence of kidney stones. However, many oxalate-degrading bacteria are susceptible to antibiotics and the use of oxalate-degrading probiotics has only led to an ephemeral reduction in urinary oxalate. The objective of the current study was to determine the efficacy of using whole-community microbial transplants from a wild mammalian herbivore, Neotoma albigula, to increase oxalate degradation over the long term in the laboratory rat, Rattus norvegicus. We quantified the change in total oxalate degradation in lab rats immediately after microbial transplants and at 2- and 9-month intervals following microbial transplants. Additionally, we tracked the fecal microbiota of the lab rats, with and without microbial transplants, using high-throughput Illumina sequencing of a hyper-variable region of the 16S rRNA gene. Microbial transplants resulted in a significant increase in oxalate degradation, an effect that persisted 9 months after the initial transplants. Functional persistence was corroborated by the transfer, and persistence of a group of bacteria previously correlated with oxalate consumption in N. albigula, including an anaerobic bacterium from the genus Oxalobacter known for its ability to use oxalate as a sole carbon source. The results of this study indicate that whole-community microbial transplants are an effective means for the persistent colonization of oxalate-degrading bacteria in the mammalian gut.
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影响因子：
13.2
作者：
Hoppe, B;von Unruh, GE;Hesse, A
通讯作者：
Hesse, A
影响因子：
2.9
作者：
Grehan, Martin J.;Borody, Thomas Julius;Wettstein, Antony
通讯作者：
Wettstein, Antony
DOI：
10.1152/ajpgi.00434.2010
发表时间：
2011-03-01
影响因子：
4.5
作者：
Hatch, Marguerite;Gjymishka, Altin;Freel, Robert W.
通讯作者：
Freel, Robert W.
影响因子：
13.6
作者：
Kaufman, David W.;Kelly, Judith P.;Cave, David R.
通讯作者：
Cave, David R.
影响因子：
4.4
作者：
DANIEL, SL;HARTMAN, PA;ALLISON, MJ
通讯作者：
ALLISON, MJ