Transcriptome profile of Trichoderma harzianum IOC-3844 induced by sugarcane bagasse.
Transcriptome profile of Trichoderma harzianum IOC-3844 induced by sugarcane bagasse.
复制标题
DOI：
10.1371/journal.pone.0088689
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Souza AP
中科院分区：
文献类型：
--
作者：
Horta MA;Vicentini R;Delabona Pda S;Laborda P;Crucello A;Freitas S;Kuroshu RM;Polikarpov I;Pradella JG;Souza AP
通过对哈茨木霉降解生物量的转录组进行分析，可以鉴定在酶解过程中有潜在应用的基因序列。本研究利用RNA-seq技术对哈茨木霉IOC-3844的转录组进行了分析。测序为下游分析产生了14.7英镑。从头组装产生32,396个重叠群，提交进行鉴定并根据其身份进行分类。这一分析使我们能够确定参与纤维素和半纤维素降解的哈茨木霉的主要基因集，以及参与生物质解聚的辅助基因。对表达水平的另一项分析确定了一组在不同条件下上调的碳水化合物活性酶。本研究为今后关于生物质降解的研究提供了有价值的信息，并有助于更好地了解参与这一过程的基因的作用。
Profiling the transcriptome that underlies biomass degradation by the fungus Trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. In the present study, the transcriptome of T. harzianum IOC-3844 was analyzed using RNA-seq technology. The sequencing generated 14.7 Gbp for downstream analyses. De novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. This analysis allowed us to define a principal set of T. harzianum genes that are involved in the degradation of cellulose and hemicellulose and the accessory genes that are involved in the depolymerization of biomass. An additional analysis of expression levels identified a set of carbohydrate-active enzymes that are upregulated under different conditions. The present study provides valuable information for future studies on biomass degradation and contributes to a better understanding of the role of the genes that are involved in this process.
登录
查看更多内容
影响因子：
14.9
作者：
Kanehisa M;Goto S;Sato Y;Furumichi M;Tanabe M
通讯作者：
Tanabe M
影响因子：
2.8
作者：
Fang, Xu;Yano, Shinichi;Sawayama, Shigeki
通讯作者：
Sawayama, Shigeki
影响因子：
5.7
作者：
DAVIES, G;HENRISSAT, B
通讯作者：
HENRISSAT, B
影响因子：
11.4
作者：
JONES, JDG;DUNSMUIR, P;BEDBROOK, J
通讯作者：
BEDBROOK, J
影响因子：
6.8
作者：
Henrissat, B;Davies, G
通讯作者：
Davies, G