Annotation and classification of the bovine T cell receptor delta genes.
Annotation and classification of the bovine T cell receptor delta genes.
复制标题
DOI：
10.1186/1471-2164-11-100
复制
发表时间：
2010-02-09
期刊：
影响因子：
4.4
通讯作者：
Baldwin CL
中科院分区：
文献类型：
--
作者：
Herzig CT;Lefranc MP;Baldwin CL
γδT细胞和αβT细胞在与其T细胞受体相互作用的抗原类型上不同；γδT细胞抗原不一定是多肽，也不存在于MHC上。牛被认为是“γδT细胞高”物种，这表明它们循环中的γδT细胞比例高于人类和小鼠等“γδT细胞低”物种。在这里描述的研究开始之前，关于编码这种γδT细胞高物种的T细胞受体三角链的基因的信息有限。通过对牛(Bos Taurus)基因组Btau_3.1组装的注释，发现存在56个不同的T细胞受体增量(TRD)可变(V)基因，其中52个属于TRDV1亚群，并与T细胞受体α可变(TRAV)基因共混。此外，还发现了两个属于TRDV2亚组的基因，以及单一的TRDV3和TRDV4基因。我们证实了5个多样性(D)基因、3个连接(J)基因和一个恒定(C)基因的存在，并描述了TRD基因座的组织结构。TRDV4基因位于C基因下游，并处于反向转录方向，与其在人类和小鼠中的同源基因一致。对可变区基因的表达进行了评估，结果表明一到五个D基因可以整合到一个转录本中。最后，我们根据它们的关联性将牛和羊的TRDV1基因分组。与“γδT细胞低”物种的基因组相比，牛的基因组包含了大量和多样化的trd基因。这表明，在牛γδ中，T细胞在免疫功能中发挥着更重要的作用，因为它们被预测会结合更多种类的抗原。
γδ T cells differ from αβ T cells with regard to the types of antigen with which their T cell receptors interact; γδ T cell antigens are not necessarily peptides nor are they presented on MHC. Cattle are considered a "γδ T cell high" species indicating they have an increased proportion of γδ T cells in circulation relative to that in "γδ T cell low" species such as humans and mice. Prior to the onset of the studies described here, there was limited information regarding the genes that code for the T cell receptor delta chains of this γδ T cell high species. By annotating the bovine (Bos taurus) genome Btau_3.1 assembly the presence of 56 distinct T cell receptor delta (TRD) variable (V) genes were found, 52 of which belong to the TRDV1 subgroup and were co-mingled with the T cell receptor alpha variable (TRAV) genes. In addition, two genes belonging to the TRDV2 subgroup and single TRDV3 and TRDV4 genes were found. We confirmed the presence of five diversity (D) genes, three junctional (J) genes and a single constant (C) gene and describe the organization of the TRD locus. The TRDV4 gene is found downstream of the C gene and in an inverted orientation of transcription, consistent with its orthologs in humans and mice. cDNA evidence was assessed to validate expression of the variable genes and showed that one to five D genes could be incorporated into a single transcript. Finally, we grouped the bovine and ovine TRDV1 genes into sets based on their relatedness. The bovine genome contains a large and diverse repertoire of TRD genes when compared to the genomes of "γδ T cell low" species. This suggests that in cattle γδ T cells play a more important role in immune function since they would be predicted to bind a greater variety of antigens.
登录
查看更多内容
影响因子：
3.2
作者：
Blumerman, Seth L.;Herzig, Carolyn T. A.;Baldwin, Cynthia L.
通讯作者：
Baldwin, Cynthia L.
影响因子：
56.9
作者：
Adams, EJ;Chien, YH;Garcia, KC
通讯作者：
Garcia, KC
影响因子：
11.4
作者：
HEIN, WR;DUDLER, L
通讯作者：
DUDLER, L
影响因子：
3.2
作者：
Herzig, Carolyn T. A.;Blumerman, Seth L.;Baldwin, Cynthia L.
通讯作者：
Baldwin, Cynthia L.
影响因子：
30.5
作者：
Adams, Erin J.;Strop, Pavel;Garcia, K. Christopher
通讯作者：
Garcia, K. Christopher