Identification of TLR2/TLR6 signalling lactic acid bacteria for supporting immune regulation.
Identification of TLR2/TLR6 signalling lactic acid bacteria for supporting immune regulation.
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DOI：
10.1038/srep34561
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发表时间：
2016-10-06
影响因子：
4.6
通讯作者：
de Vos P
中科院分区：
文献类型：
--
作者：
Ren C;Zhang Q;de Haan BJ;Zhang H;Faas MM;de Vos P
虽然许多乳酸菌（LAB）影响消费者的免疫状态，但还不完全清楚这是如何建立的。细菌细胞壁组分和Toll样受体（TLR）之间的细菌-宿主相互作用已被认为发挥重要作用。在这里，我们研究了LAB与报告的健康影响和TLR之间的相互作用。通过使用表达单个或组合TLR的细胞系，我们表明LAB可以通过TLR依赖性和非依赖性途径进行信号传导。这些菌株仅刺激而不抑制TLR。结果表明，L. plantarum CCFM634，L. plantarum CCFM734，L. fermentum CCFM381，L. acidophilus CCFM 137和S.嗜热菌CCFM 218刺激TLR 2/TLR 6。TLR 2/TLR 6在免疫调节过程中是必不可少的，并且对预防疾病有意义。TLR 2/TLR 6刺激的特异性用阻断抗体证实。还通过评估细菌刺激的THP 1衍生的巨噬细胞中的IL-10和IL-6分泌模式研究了LAB的免疫调节特性，这证实了LAB的种属和菌株特异性作用。通过这项研究，我们提供了新的见解，在LAB特定的宿主-微生物相互作用。我们的数据表明，模式识别受体（如TLR）之间的相互作用是物种和菌株特异性的，并强调了选择特定菌株以促进特定健康效应的重要性。
Although many lactic acid bacteria (LAB) influence the consumer’s immune status it is not completely understood how this is established. Bacteria-host interactions between bacterial cell-wall components and toll-like receptors (TLRs) have been suggested to play an essential role. Here we investigated the interaction between LABs with reported health effects and TLRs. By using cell-lines expressing single or combination of TLRs, we show that LABs can signal via TLR-dependent and independent pathways. The strains only stimulated and did not inhibit TLRs. We found that several strains such as L. plantarum CCFM634, L. plantarum CCFM734, L. fermentum CCFM381, L. acidophilus CCFM137, and S. thermophilus CCFM218 stimulated TLR2/TLR6. TLR2/TLR6 is essential in immune regulatory processes and of interest for prevention of diseases. Specificity of the TLR2/TLR6 stimulation was confirmed with blocking antibodies. Immunomodulatory properties of LABs were also studied by assessing IL-10 and IL-6 secretion patterns in bacteria-stimulated THP1-derived macrophages, which confirmed species and strain specific effects of the LABs. With this study we provide novel insight in LAB specific host-microbe interactions. Our data demonstrates that interactions between pattern recognition receptors such as TLRs is species and strain specific and underpins the importance of selecting specific strains for promoting specific health effects.
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影响因子：
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作者：
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