Respiratory viruses in adults with community-acquired pneumonia.
Respiratory viruses in adults with community-acquired pneumonia.
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患有社区获得性肺炎的成年人的呼吸道病毒。
DOI：
10.1378/chest.09-2717
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发表时间：
2010-10
期刊：
影响因子：
9.6
通讯作者：
Lieberman D
中科院分区：
文献类型：
--
作者：
Lieberman D;Shimoni A;Shemer-Avni Y;Keren-Naos A;Shtainberg R;Lieberman D
核酸扩增技术的使用增加了社区获得性肺炎（CAP）成人患者呼吸道病毒（RV）的鉴定。本研究的目的是使用三种不同的采样方法识别CAP患者的RV，并比较两个对照组的CAP病毒比例和类型。研究人群包括183例成人CAP患者、450例对照组和201例非肺炎性下呼吸道感染（NPLRTI）患者。通过口咽拭子、鼻咽拭子和鼻咽冲洗对每名参与者进行采样，并通过基于多重TaqMan水解探针的实时聚合酶链反应（Integrated DNA Technology;科拉尔维尔，IA）对样本进行12种RV检测。58例CAP患者（31.7%）至少有一个RV，而对照组为32例（7.1%），NPLRTI患者为104例（51.7%）（分别为P <0.01和P <0.01）。冠状病毒在24例（13.1%）CAP患者中被发现，而对照组为17例（3.8%），NPLRTI患者为21例（10.4%）。分别在13例（7.1%）、4例（0.9%）和7例（3.5%）CAP患者、对照受试者和NPLRTI患者中鉴定出呼吸道合胞病毒; 9例（4.9%）、9例（2.0%）和15例（7.5%）鼻病毒; 8例（4.4%）、2例（0.4%）和63例（31.3%）流感病毒。RV参与CAP的比例高于以往报道。在健康受试者中确定的RV比例显著低于CAP，但它不是零，在解释患者中的相应比例时应进行权衡。
Use of nucleic acid amplification techniques has increased the identification of respiratory viruses (RVs) in adult patients with community-acquired pneumonia (CAP). The objectives of the present study were to identify RV in patients with CAP using three different sampling methods and to compare CAP virus proportions and types with two comparison groups. The study population included 183 adult patients with CAP, 450 control subjects, and 201 patients with nonpneumonic lower respiratory tract infection (NPLRTI). Each participant was sampled by oropharyngeal swab, nasopharyngeal swab, and nasopharyngeal washing, and the samples were tested for detection of 12 RVs by multiplex TaqMan Hydrolysis probe-based real-time polymerase chain reaction (Integrated DNA Technology; Coralville, IA). At least one RV was identified in 58 patients with CAP (31.7%) compared with 32 (7.1%) in control subjects and 104 (51.7%) in patients with NPLRTI (P < .01 and P < .01, respectively). Coronaviruses were identified in 24 (13.1%) patients with CAP, compared with 17 (3.8%) in control subjects, and 21 (10.4%) patients with NPLRTI. Respiratory syncytial virus was identified in 13 (7.1%), four (0.9%), and seven (3.5%); rhinovirus in nine (4.9%), nine (2.0%), and 15 (7.5%); and influenza virus in eight (4.4%), two (0.4%), and 63 (31.3%) patients with CAP, control subjects, and patients with NPLRTI, respectively. The proportion of RV involvement in CAP is higher than previously reported. The proportion of RV identified in healthy subjects is significantly lower than in CAP, but it is not zero and should be weighed when interpreting corresponding proportions among patients.
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影响因子：
9.4
作者：
Lieberman, David;Lieberman, Devora;Shemer-Avni, Yonat
通讯作者：
Shemer-Avni, Yonat
影响因子：
--
作者：
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通讯作者：
Peeters, Marcel F.
影响因子：
11.8
作者：
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通讯作者：
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影响因子：
15.9
作者：
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通讯作者：
Bakaletz, Lauren O.
影响因子：
6.4
作者：
van Elden, LJR;van Loon, AM;Nijhuis, M
通讯作者：
Nijhuis, M