Expression of acetylated histone 3 in the spinal cord and the effect of morphine on inflammatory pain in rats.
Expression of acetylated histone 3 in the spinal cord and the effect of morphine on inflammatory pain in rats.
复制标题
乙酰化组蛋白3在大鼠脊髓中的表达及吗啡对大鼠炎性痛的影响
DOI：
10.3969/j.issn.1673-5374.2012.07.007
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发表时间：
2012-03-05
影响因子：
6.1
通讯作者：
Luo X
中科院分区：
文献类型：
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作者：
Li H;Li C;Dai R;Shi X;Xu J;Zhang J;Zhou X;Li Z;Luo X
本研究采用完全弗氏佐剂后爪注射法制备大鼠炎症性疼痛模型，用免疫组织化学方法检测乙酰化组蛋白3在脊髓背角的表达。注射后1d，脊髓背角神经元乙酰化组蛋白3的表达显著减少。然而，在第7天，注射佐剂的大鼠的表达恢复。乙酰化组蛋白3标记存在于背角神经元中，而在星形胶质细胞和小胶质细胞中更为丰富。乙酰化组蛋白3表达的恢复与该蛋白的表达从神经元向神经胶质细胞转移有关。注射吗啡后1d，脊髓背角神经元和神经胶质细胞中乙酰化组蛋白3的表达显著上调，尤其是星形胶质细胞，阻止了这种短暂的下调。我们的结果表明，炎性疼痛在佐剂注射后的早期阶段诱导脊髓背角乙酰化组蛋白3的一过性下调，这种作用可以被吗啡逆转。因此，乙酰化组蛋白3的下调可能参与了炎症性疼痛的发生。
In this study, a rat model of inflammatory pain was produced by injecting complete Freund's adjuvant into the hind paw, and the expression of acetylated histone 3 in the spinal cord dorsal horn was examined using immunohistochemical staining. One day following injection, there was a dramatic decrease in acetylated histone 3 expression in spinal cord dorsal horn neurons. However, on day 7, expression recovered in adjuvant-injected rats. While acetylated histone 3 labeling was present in dorsal horn neurons, it was more abundant in astrocytes and microglial cells. The recovery of acetylated histone 3 expression was associated with a shift in expression of the protein from neurons to glial cells. Morphine injection significantly upregulated the expression of acetylated histone 3 in spinal cord dorsal horn neurons and glial cells 1 day after injection, especially in astrocytes, preventing the transient downregulation. Our results indicate that inflammatory pain induces a transient downregulation of acetylated histone 3 in the spinal cord dorsal horn at an early stage following adjuvant injection, and that this effect can be reversed by morphine. Thus, the downregulation of acetylated histone 3 may be involved in the development of inflammatory pain.
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影响因子：
7.4
作者：
Obara, Ilona;Parkitna, Jan Rodriguez;Przewlocki, Ryszard
通讯作者：
Przewlocki, Ryszard
影响因子：
10.5
作者：
Berger, Shelley L.;Kouzarides, Tony;Shilatifard, Ali
通讯作者：
Shilatifard, Ali
影响因子：
7.4
作者：
Polgár, E;Hughes, DI;Todd, AJ
通讯作者：
Todd, AJ
影响因子：
2.7
作者：
Yang, Jianli;Yu, Jingjie;Lu, Lin
通讯作者：
Lu, Lin
DOI：
10.1038/nrg2485
发表时间：
2009-01
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
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