Synergistic mechanism of Ag(+)-Zn(2+) in anti-bacterial activity against Enterococcus faecalis and its application against dentin infection.
Synergistic mechanism of Ag(+)-Zn(2+) in anti-bacterial activity against Enterococcus faecalis and its application against dentin infection.
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Ag( )-Zn(2 )对粪肠球菌的协同抗菌机制及其在牙本质感染中的应用
DOI：
10.1186/s12951-018-0336-3
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发表时间：
2018-01-31
影响因子：
10.2
通讯作者：
Fan B
中科院分区：
文献类型：
--
作者：
Fan W;Sun Q;Li Y;Tay FR;Fan B
Ag+和Zn 2+已经组合使用以获得增强的抗菌效果和低细胞毒性。尽管如此，仍不清楚Zn 2+如何与Ag+协同抗菌活性。研究了Ag+和Zn 2+协同抗菌作用的协同方式和最佳配比。faecalis之间的协同作用，这种协同作用背后的可能机制，以及最佳Ag+-Zn 2+协同模式对大肠杆菌的初步应用。牙本质上的粪生物膜。在细菌和生物膜上测试了一系列Ag+-Zn 2+原子结合比。粪菌对牙本质的抑菌效果，并确定最佳配比。并对不同Ag+-Zn ~（2+）原子比的MC 3 T3-E1细胞进行细胞毒性试验。Zn 2+在Ag+-Zn 2+协同工作中的作用通过Zn 2+预处理研究和膜电位-渗透性测量来评估。结果表明，Ag+-Zn 2+的协同增效作用是Zn 2+依赖性的，Ag+：Zn 2+原子比为1：9和1：12时，对寄生菌和生物膜寄生菌的抗菌能力最强。粪便。这种协同工作可能是由于E. faecalis细胞膜上的Zn ~（2+）。Ag+-Zn ~（2+）原子比为1：9和1：12时的细胞毒性远低于2%洗必泰。Ag ~+-Zn ~（2+）原子比为1：9和1：12的菌株对大肠杆菌的抗菌能力相近。粪菌生物膜对牙本质的细胞毒性明显低于2%洗必泰。含有最佳Ag+-Zn 2+原子比高于1：6（如1：9或1：12）的新药物可被开发用于抗E.牙齿或人体任何其他部位的根管中的粪菌感染。
Ag+ and Zn2+ have already been used in combinations to obtain both enhanced antibacterial effect and low cytotoxicity. Despite this, it is still unclear how the Zn2+ co-works with Ag+ in the synergistic antibacterial activity. The main purposes of this study were to investigate the co-work pattern and optimum ratio between Ag+ and Zn2+ in their synergistic antibacterial activity against E. faecalis, the possible mechanisms behind this synergy and the primary application of optimum Ag+–Zn2+ co-work pattern against the E. faecalis biofilm on dentin. A serial of Ag+–Zn2+ atomic combination ratios were tested on both planktonic and biofilm-resident E. faecalis on dentin, their antibacterial efficiency was calculated and optimum ratio determined. And the cytotoxicity of various Ag+–Zn2+ atomic ratios was tested on MC3T3-E1 Cells. The role of Zn2+ in Ag+–Zn2+co-work was evaluated using a Zn2+ pretreatment study and membrane potential—permeability measurement. The results showed that the synergistically promoted antibacterial effect of Ag+–Zn2+ combinations was Zn2+ amount-dependent with the 1:9 and 1:12 Ag+–Zn2+ atomic ratios showing the most powerful ability against both planktonic and biofilm-resident E. faecalis. This co-work could likely be attributed to the depolarization of E. faecalis cell membrane by the addition of Zn2+. The cytotoxicity of the Ag+–Zn2+ atomic ratios of 1:9 and 1:12 was much lower than 2% chlorhexidine. The Ag+–Zn2+ atomic ratios of 1:9 and 1:12 demonstrated similar strong ability against E. faecalis biofilm on dentin but much lower cytotoxicity than 2% chlorhexidine. New medications containing optimum Ag+–Zn2+ atomic ratios higher than 1:6, such as 1:9 or 1:12, could be developed against E. faecalis infection in root canals of teeth or any other parts of human body.
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影响因子：
5
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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