Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging In Vivo.
Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging In Vivo.
复制标题
DOI：
10.1021/jacs.2c02320
复制
发表时间：
2022-08-10
影响因子：
15
通讯作者：
Ai, Hui-wang
中科院分区：
文献类型：
--
作者：
Xiong, Ying;Zhang, Yiyu;Li, Zefan;Reza, Md Shamim;Li, Xinyu;Tian, Xiaodong;Ai, Hui-wang
The NanoLuc luciferase (NLuc) and its furimazine (FRZ) substrate have revolutionized bioluminescence (BL) assays and imaging. However, the use of the NLuc-FRZ luciferase-luciferin pair for mammalian tissue imaging is hindered by the low tissue penetration of the emitting blue photons. Here, we present the development of an NLuc mutant, QLuc, which catalyzes the oxidation of a synthetic QTZ luciferin for bright and red-shifted emission peaking at ~ 585 nm. Compared to other small single-domain NLuc mutants, this amber-light-emitting luciferase exhibited improved performance for imaging deep-tissue targets in live mice. Leveraging this novel bioluminescent reporter, we further pursued in vivo immunobioluminescence imaging (immunoBLI), which used a fusion protein of a single-chain variable antibody fragment (scFv) and QLuc for molecular imaging of tumor-associated antigens in a xenograft mouse model. As one of the most red-shifted NLuc variants, we expect QLuc to find broad applications in noninvasive mammalian imaging. Moreover, the immunoBLI method complements immunofluorescence imaging and immuno-positron emission tomography (immunoPET), serving as a convenient and nonradioactive molecular imaging tool for animal models in basic and preclinical research.
登录
查看更多内容
影响因子：
48
作者：
Loening, Andreas Markus;Wu, Anna M.;Gambhir, Sanjiv Sam
通讯作者：
Gambhir, Sanjiv Sam
DOI：
10.1007/s00259-021-05439-4
发表时间：
2022-03
影响因子：
9.1
作者：
Amadeo F;Plagge A;Chacko A;Wilm B;Hanson V;Liptrott N;Murray P;Taylor A
通讯作者：
Taylor A
影响因子：
3.4
作者：
Fu R;Carroll L;Yahioglu G;Aboagye EO;Miller PW
通讯作者：
Miller PW
DOI：
10.4049/jimmunol.2100274
发表时间：
2021-09-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Islam A;Pishesha N;Harmand TJ;Heston H;Woodham AW;Cheloha RW;Bousbaine D;Rashidian M;Ploegh HL
通讯作者：
Ploegh HL
影响因子：
7.8
作者：
Adams, Spencer T., Jr.;Miller, Stephen C.
通讯作者：
Miller, Stephen C.