Proximal tissues and patterned neurite outgrowth at the lumbosacral level of the chick embryo: partial and complete deletion of the somite.
Proximal tissues and patterned neurite outgrowth at the lumbosacral level of the chick embryo: partial and complete deletion of the somite.
复制标题
鸡胚腰骶水平的近端组织和图案化神经突生长：体节的部分和完全缺失。
DOI：
10.1016/0012-1606(88)90314-4
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发表时间：
1988
影响因子：
2.7
通讯作者：
Tosney,KW
中科院分区：
文献类型：
--
作者：
Tosney,KW
在部分或全部去除鸡体细胞中胚层后，观察了图案化轴突生长和背根神经节（DRG）形成的发育。由于皮肌节不是必需的，肢体肌肉的完整补充得以发育，神经模式的改变可归因于硬骨节的缺失。当体细胞组织被完全去除，轴突延伸和DRG形成，但在一个不分段的模式。因此，体节不引起轴突的生长或DRG前体的迁移，它不是一个manditory基质，它不是DRG凝聚所必需的。这些结果表明，后巩膜是相对抑制入侵，抑制释放时，巩膜是缺席。当体节被部分删除，轴突分割没有失去比例与去除的量，这表明，可能会与巩膜体积（如扩散线索）的属性，不发挥主要作用。相反，脊神经失去分割时，腹侧巩膜被删除，无论是否背侧巩膜被删除。这强烈表明轴突分割是由生长锥与细胞外基质或硬节细胞表面之间的直接相互作用造成的。当腹侧硬节缺失时，DRG趋向于正常分段，当背内侧硬节缺失时，DRG趋向于失去分段，但不能排除DRG分段与硬节体积的协调损失。然而，很明显，轴突和DRG分割是独立的。对脊索相对于脊髓移位的胚胎亚组的观察表明，脊索周围的腹内侧硬结抑制轴突前进。后和腹内侧硬节被假设为轴突生长的障碍，由于它们共同的软骨发育的一些功能。还检查了特定的神经支配模式。当脊索向控制侧移位时，这一侧的轴突产生并纠正投射误差，表明脊索可以影响轴突通路选择的精确性。与此相反，运动轴突进入肢体上的所有操作侧支配肌肉与他们的正常精度，尽管没有体节和轴突分割。因此，体节和脊神经分段的过程在很大程度上与运动神经元投射的特异性无关。
The development of patterned axon outgrowth and dorsal root ganglion (DRG) formation was examined after partially or totally removing chick somitic mesoderm. Since the dermamyotome is not essential and a full complement of limb muscles developed, alterations in neural patterns could be ascribed to deletion of sclerotome. When somitic tissue was completely removed, axons extended and DRG formed, but in an unsegmented pattern. Therefore the somite does not elicit outgrowth of axons or migration of DRG precursors, it is not a manditory substratum and it is not required for DRG condensation. These results suggest that posterior sclerotome is relatively inhibitory to invasion, an inhibition that is released when sclerotome is absent. When somites were partially deleted, axonal segmentation was not lost proportionally with the amount of sclerotome removed, suggesting that properties that may vary with sclerotome volume (such as diffusible cues) do not play a primary role. Instead, spinal nerves lost segmentation only when ventral sclerotome was deleted, regardless of whether dorsal sclerotome was or was not removed. This strongly suggests that axonal segmentation is imposed by direct interactions between growth cones and extracellular matrices or surfaces of sclerotome cells. While DRG tended to be normally segmented when ventral sclerotome was deleted and to lose segmentation when dorsomedial sclerotome was absent, a coordinate loss of DRG segmentation with sclerotome volume could not be ruled out. However it is clear that axonal and DRG segmentation are independent. Observations on a subset of embryos in which the notochord was displaced relative to the spinal cord suggest that the ventromedial sclerotome surrounding the notochord inhibits axon advance. Posterior and ventromedial sclerotome are hypothesized to act as barriers to axon outgrowth due to some feature of their common cartilaginous development. Specific innervation patterns were also examined. When the notochord was displaced toward the control limb, axons on this side made and corrected projection errors, suggesting that the notochord can influence the precision of axonal pathway selection. In contrast, motor axons that entered the limb on all operated sides innervated muscle with their normal precision despite the absence of the somite and axonal segmentation. Therefore, the somite and the process of spinal nerve segmentation are largely irrelevant to the specificity of motoneuron projection.
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影响因子：
2.7
作者：
Kathryn W. Tosney
通讯作者：
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DOI：
10.1523/jneurosci.03-09-01760.1983
发表时间：
1983
期刊：
Progress in clinical and biological research
影响因子：
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作者：
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影响因子：
2.7
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1113/jphysiol.1980.sp013262
发表时间：
1980
期刊：
The Journal of Physiology
影响因子：
--
作者：
C. Lance‐Jones;L. Landmesser
通讯作者：
L. Landmesser