Salivary Microbiome Diversity in Caries-Free and Caries-Affected Children.
Salivary Microbiome Diversity in Caries-Free and Caries-Affected Children.
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无龋齿和受龋齿影响的儿童的唾液微生物组多样性。
DOI：
10.3390/ijms17121978
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发表时间：
2016-11-25
影响因子：
5.6
通讯作者：
Lo EC
中科院分区：
文献类型：
--
作者：
Jiang S;Gao X;Jin L;Lo EC
龋齿（蛀牙）是一种传染病。从微生物学角度来看，其病因学尚未完全了解。本研究的特点的微生物植物群的多样性，在儿童唾液中有和没有龋齿。选取3-4岁患龋儿童和无龋儿童各20例。从每个儿童收集未刺激的唾液（2 mL），并提取总微生物基因组DNA。产生细菌16 S rRNA基因的V3-V4高变区的DNA扩增子并进行Illumina Miseq测序。结果表明，唾液中细菌有17门、26纲、40目、80科、151属、310种。患龋儿童和无龋儿童的微生物组多样性无显著差异（p > 0.05）。有龋组中几种细菌（龋齿罗氏菌、格雷文氏放线菌、韦荣氏球菌口腔分类群780、唾液普氏菌和变形链球菌）的相对丰度高于无龋组（p < 0.05）。无龋儿童牙周梭杆菌和口腔纤毛菌FP 036的检出率高于患龋儿童（p < 0.05）。无龋儿童和患龋儿童的唾液微生物组特征相似。唾液中某些细菌的数量，如R。dentocariosa和F.牙周炎可能是有用的筛选/评估儿童的风险发展龋齿。
Dental caries (tooth decay) is an infectious disease. Its etiology is not fully understood from the microbiological perspective. This study characterizes the diversity of microbial flora in the saliva of children with and without dental caries. Children (3–4 years old) with caries (n = 20) and without caries (n = 20) were recruited. Unstimulated saliva (2 mL) was collected from each child and the total microbial genomic DNA was extracted. DNA amplicons of the V3-V4 hypervariable region of the bacterial 16S rRNA gene were generated and subjected to Illumina Miseq sequencing. A total of 17 phyla, 26 classes, 40 orders, 80 families, 151 genera, and 310 bacterial species were represented in the saliva samples. There was no significant difference in the microbiome diversity between caries-affected and caries-free children (p > 0.05). The relative abundance of several species (Rothia dentocariosa, Actinomyces graevenitzii, Veillonella sp. oral taxon 780, Prevotella salivae, and Streptococcus mutans) was higher in the caries-affected group than in the caries-free group (p < 0.05). Fusobacterium periodonticum and Leptotrichia sp. oral clone FP036 were more abundant in caries-free children than in caries-affected children (p < 0.05). The salivary microbiome profiles of caries-free and caries-affected children were similar. Salivary counts of certain bacteria such as R. dentocariosa and F. periodonticum may be useful for screening/assessing children’s risk of developing caries.
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影响因子：
15.5
作者：
Fadrosh DW;Ma B;Gajer P;Sengamalay N;Ott S;Brotman RM;Ravel J
通讯作者：
Ravel J
影响因子：
3.6
作者：
Ling, Zongxin;Kong, Jianming;Xiang, Charlie
通讯作者：
Xiang, Charlie
影响因子：
4.2
作者：
Raja, M.;Hannan, A.;Ali, K.
通讯作者：
Ali, K.
影响因子：
9.4
作者：
Tanner, A. C. R.;Mathney, J. M. J.;Dewhirst, F. E.
通讯作者：
Dewhirst, F. E.
DOI：
10.1099/ijs.0.02876-0
发表时间：
2004-05-01
影响因子：
2.8
作者：
Sakamoto, M;Suzuki, M;Benno, Y
通讯作者：
Benno, Y