Sex Differences in MicroRNA Expression and Cardiometabolic Risk Factors in Hispanic Adolescents with Obesity.
Sex Differences in MicroRNA Expression and Cardiometabolic Risk Factors in Hispanic Adolescents with Obesity.
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DOI：
10.1016/j.jpeds.2021.03.070
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Cuda S
中科院分区：
文献类型：
--
作者：
Karere GM;Cox LA;Bishop AC;South AM;Shaltout HA;Mercado-Deane MG;Cuda S
评估西班牙裔肥胖青少年microRNA（miRNA）表达、人体测量指标和心脏代谢危险因素的性别差异。对68名（60%为男性）西班牙裔肥胖青少年进行横断面研究，年龄13-17岁，从儿科体重管理诊所招募。我们使用小RNA测序来鉴定差异表达的循环miRNA。我们使用Influencity Pathway Analysis和大卫生物信息学资源工具来鉴定这些miRNA的靶基因和富集的通路。我们使用标准程序来测量人体测量和心脏代谢因素。我们鉴定了5种在女性和男性之间不同的miRNAs（miR-24- 3 p、miR-361- 3 p、miR-3605- 5 p、miR-486- 5 p和miR-199 b-3 p）。miRNA靶点富集途径包括PI 3 K-AKT、AMPK、胰岛素抵抗、鞘脂、TGF-β、脂肪细胞脂解调节和催产素信号通路。此外，血压、骨骼肌质量、瘦体重和体脂百分比也存在性别差异。我们已经确定了与心脏代谢健康相关的西班牙裔青少年中miRNA表达的性别差异。未来的研究应集中在与肥胖病理生理相关的基因通路上的性别特异性机制作用，以支持精确的心脏代谢干预措施的发展。
To evaluate sex differences in microRNA (miRNA) expression, anthropometric measures and cardiometabolic risk factors in Hispanic adolescents with obesity. Cross-sectional study of 68 (60% male) Hispanic adolescents with obesity, aged 13–17 years, recruited from a pediatric weight management clinic. We used small RNA sequencing to identify differentially expressed circulating miRNAs. We used Ingenuity Pathway Analysis and David bioinformatic resource tools to identify target genes for these miRNAs and enriched pathways. We used standard procedures to measure anthropometric and cardiometabolic factors. We identified five miRNAs (miR-24–3p, miR-361–3p, miR-3605–5p, miR-486–5p and miR-199b-3p) that differed between females and males. miRNA targets-enriched pathways included PI3K-AKT, AMPK, insulin resistance, sphingolipid, TGF-beta, adipocyte lipolysis regulation and oxytocin signaling pathways. In addition, there were sex differences in blood pressure, skeletal muscle mass, lean body mass and percent body fat. We have identified sex differences in miRNA expression in Hispanic adolescents relevant to cardiometabolic health. Future studies should focus on sex-specific mechanistic roles of miRNAs on gene pathways associated with obesity pathophysiology to support development of precision cardiometabolic interventions.
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