Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications
Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications
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DOI：
10.3390/life12060831
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发表时间：
2022-06-02
期刊：
影响因子：
3.2
通讯作者：
Diaz-Vazquez, Liz M.
中科院分区：
文献类型：
--
作者：
Torres-Diaz, Marielys;Abreu-Takemura, Caren;Diaz-Vazquez, Liz M.
关键词：
微藻肽由于其功能特性，在医学和工业上有着广泛的应用。然而，生物样品中不天然存在的肽的快速降解是一个挑战。利用载体保护活性肽并调控其释放是提高微藻肽在生物样品中稳定性的一种策略。本研究探讨了金纳米颗粒（AuNPs）作为小球藻微藻肽（VECYGPNRPQF）载体的使用。本文还讨论了这些肽生物分子作为稳定剂在生理环境中提高AuNPs胶体稳定性的潜力。光谱（UV-VIS， DLS）和显微镜（TEM）分析证实，所采用的修饰方法产生了平均直径为15 nm的球形AuNPs。通过TEM图像和FTIR光谱证实了AuNPs成功的肽盖层。当微藻肽固定在AuNPs表面时，微藻肽的稳定性增加，这一点通过观察到的DSC热位移和胶体溶液中的高ζ电位值得到证实。通过优化AuNPs的合成，并通过各种替代方法跟踪AuNPs被肽修饰时所赋予的化学性质，合成了一种有效的基于肽的AuNPs和药物载体涂层体系。微藻肽AuNPs具有较低的生态毒性和较好的生存力。
Microalgae peptides have many medical and industrial applications due to their functional properties. However, the rapid degradation of peptides not naturally present in biological samples represents a challenge. A strategy to increase microalgae peptide stability in biological samples is to use carriers to protect the active peptide and regulate its release. This study explores the use of gold nanoparticles (AuNPs) as carriers of the Chlorella microalgae peptide (VECYGPNRPQF). The potential of these peptide biomolecules as stabilizing agents to improve the colloidal stability of AuNPs in physiological environments is also discussed. Spectroscopic (UV-VIS, DLS) and Microscopic (TEM) analyses confirmed that the employed modification method produced spherical AuNPs by an average 15 nm diameter. Successful peptide capping of AuNPs was confirmed with TEM images and FTIR spectroscopy. The stability of the microalgae peptide increased when immobilized into the AuNPs surface, as confirmed by the observed thermal shifts in DSC and high zeta-potential values in the colloidal solution. By optimizing the synthesis of AuNPs and tracking the conferred chemical properties as AuNPs were modified with the peptide via various alternative methods, the synthesis of an effective peptide-based coating system for AuNPs and drug carriers was achieved. The microalgae peptide AuNPs showed lower ecotoxicity and better viability than the regular AuNPs.
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DOI：
10.3390/molecules27051692
发表时间：
2022-03-04
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Lomelí-Rosales DA;Zamudio-Ojeda A;Reyes-Maldonado OK;López-Reyes ME;Basulto-Padilla GC;Lopez-Naranjo EJ;Zuñiga-Mayo VM;Velázquez-Juárez G
通讯作者：
Velázquez-Juárez G
影响因子：
15
作者：
Doane, Tennyson L.;Cheng, Yu;Burda, Clemens
通讯作者：
Burda, Clemens
影响因子：
5.4
作者：
Martínez Andrade KA;Lauritano C;Romano G;Ianora A
通讯作者：
Ianora A
影响因子：
7.4
作者：
GRABAR, KC;FREEMAN, RG;NATAN, MJ
通讯作者：
NATAN, MJ
DOI：
10.3390/foods10071626
发表时间：
2021-07-14
期刊：
Foods (Basel, Switzerland)
影响因子：
--
作者：
Kratzer R;Murkovic M
通讯作者：
Murkovic M