Fine-Tuning Motile Cilia and Flagella: Evolution of the Dynein Motor Proteins from Plants to Humans at High Resolution.
Fine-Tuning Motile Cilia and Flagella: Evolution of the Dynein Motor Proteins from Plants to Humans at High Resolution.
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DOI：
10.1093/molbev/msw213
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发表时间：
2016-12
影响因子：
10.7
通讯作者：
Kollmar M
中科院分区：
文献类型：
--
作者：
Kollmar M
鞭毛是与真核发生相关的关键创新。它通过调节弯曲和弯曲传播的周期来提供运动性，这被认为是由外臂动力蛋白（OAD）和内臂动力蛋白（IAD）复合物的重复模式中的七种不同动力蛋白的复杂排列控制的。电子断层扫描显示在纤毛虫，藻类和动物的轴丝重复模式的高度相似性，但在真核生物的动力蛋白序列的多样性尚未得到全面解决，并与结构数据相关。为了阐明轴丝的进化，我使用现有的测序基因组数据对动力蛋白进行了详尽的分析。来自运动域的遗传学证据允许通过8种额外的亚型来扩展当前的9种动力蛋白亚型，然而，这些亚型的分类分布受到限制。我证实了在所有真核生物的超级群体中存在九种动力蛋白，这表明它们的起源早于最后一个真核生物的共同祖先。N-末端尾部结构域的比较揭示了最有可能的轴丝动力蛋白起源的新类别，一组嵌合动力蛋白在植物/藻类和Straveliles，和独特的结构域架构和起源的最外层OAD存在于绿色藻类和纤毛虫，但不是动物。序列和结构数据的相关性表明，单头8类和9类动力蛋白定位到轴丝重复序列的远端，7类动力蛋白填充该区域到近端异二聚体IAD。在真核生物中追踪动力蛋白基因的重复表明，现存分类群和物种中鞭毛功能正在进行多样化和微调。
The flagellum is a key innovation linked to eukaryogenesis. It provides motility by regulated cycles of bending and bend propagation, which are thought to be controlled by a complex arrangement of seven distinct dyneins in repeated patterns of outer- (OAD) and inner-arm dynein (IAD) complexes. Electron tomography showed high similarity of this axonemal repeat pattern across ciliates, algae, and animals, but the diversity of dynein sequences across the eukaryotes has not yet comprehensively been resolved and correlated with structural data. To shed light on the evolution of the axoneme I performed an exhaustive analysis of dyneins using the available sequenced genome data. Evidence from motor domain phylogeny allowed expanding the current set of nine dynein subtypes by eight additional isoforms with, however, restricted taxonomic distributions. I confirmed the presence of the nine dyneins in all eukaryotic super-groups indicating their origin predating the last eukaryotic common ancestor. The comparison of the N-terminal tail domains revealed a most likely axonemal dynein origin of the new classes, a group of chimeric dyneins in plants/algae and Stramenopiles, and the unique domain architecture and origin of the outermost OADs present in green algae and ciliates but not animals. The correlation of sequence and structural data suggests the single-headed class-8 and class-9 dyneins to localize to the distal end of the axonemal repeat and the class-7 dyneins filling the region up to the proximal heterodimeric IAD. Tracing dynein gene duplications across the eukaryotes indicated ongoing diversification and fine-tuning of flagellar functions in extant taxa and species.
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影响因子：
3.7
作者：
Hou, Yuqing;Witman, George B.
通讯作者：
Witman, George B.
影响因子：
4
作者：
Hook, Peter;Vallee, Richard B.
通讯作者：
Vallee, Richard B.
通过剪接体介导的反式剪接切除肠贾第鞭毛虫基因组中的分裂内含子
影响因子：
9.2
作者：
Kamikawa, Ryoma;Inagaki, Yuji;Hashimoto, Tetsuo
通讯作者：
Hashimoto, Tetsuo
DOI：
10.1196/annals.1389.017
发表时间：
2007-01-01
期刊：
REPRODUCTIVE BIOMECHANICS
影响因子：
--
作者：
Inaba, Kazuo
通讯作者：
Inaba, Kazuo
影响因子：
--
作者：
ASAI, DJ
通讯作者：
ASAI, DJ