Tumor-associated macrophages (TAMs) form an interconnected cellular supportive network in anaplastic thyroid carcinoma.
Tumor-associated macrophages (TAMs) form an interconnected cellular supportive network in anaplastic thyroid carcinoma.
复制标题
DOI：
10.1371/journal.pone.0022567
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Dupuy C
中科院分区：
文献类型：
--
作者：
Caillou B;Talbot M;Weyemi U;Pioche-Durieu C;Al Ghuzlan A;Bidart JM;Chouaib S;Schlumberger M;Dupuy C
最近报道了甲状腺癌患者中肿瘤相关巨噬细胞（TAM）密度增加与生存率降低之间的关系。在这些肿瘤中，间变性甲状腺癌（ATC）是人类最具侵袭性的实体肿瘤之一。TAM（M2型）已被公认为促进肿瘤生长。本研究的目的是用免疫组织化学方法分析27例ATC中TAM的存在。使用了几种巨噬细胞标志物，如NADPH氧化酶复合物N 0X 2-p22 phox、CD 163和CD 68。免疫染色显示TAM占所有ATC中有核细胞的50%以上。此外，这些标记物允许鉴定细长的细分支细胞质延伸，赋予这些细胞上的“小胶质细胞样”外观，我们称之为“分支TAM”（RTAMs）。相反，癌细胞完全阴性。细胞基质被高度简化，因为除了癌细胞和血管，RTAMs是唯一的其他细胞成分。RTAMs均匀分布并与癌细胞混合，并通过其分支与其他RTAMs直接接触。此外，RTAMs显示连接蛋白Cx43的强免疫染色。长链的相互连接的RTAMs从血管周围的集群和分散在肿瘤实质。当表达时，葡萄糖转运蛋白Glut 1在RTAMs和血管中被发现，但很少在癌细胞中被发现。ATC显示出与混合的癌细胞直接接触的相互连接的RTAM的非常密集的网络。据我们所知，这是第一次在恶性肿瘤中描述这样的网络。该网络在我们研究的所有病例中都有发现，并且似乎是ATC特有的，因为它在分化型甲状腺癌标本中没有发现。总之，这些结果表明，RTAMs网络是直接相关的疾病的侵略性，通过代谢和营养功能，仍有待确定。
A relationship between the increased density of tumor-associated macrophages (TAMs) and decreased survival was recently reported in thyroid cancer patients. Among these tumors, anaplastic thyroid cancer (ATC) is one of the most aggressive solid tumors in humans. TAMs (type M2) have been recognized as promoting tumor growth. The purpose of our study was to analyze with immunohistochemistry the presence of TAMs in a series of 27 ATC. Several macrophages markers such as NADPH oxidase complex NOX2-p22phox, CD163 and CD 68 were used. Immunostainings showed that TAMs represent more than 50% of nucleated cells in all ATCs. Moreover, these markers allowed the identification of elongated thin ramified cytoplasmic extensions, bestowing a “microglia-like” appearance on these cells which we termed “Ramified TAMs” (RTAMs). In contrast, cancer cells were totally negative. Cellular stroma was highly simplified since apart from cancer cells and blood vessels, RTAMs were the only other cellular component. RTAMs were evenly distributed and intermingled with cancer cells, and were in direct contact with other RTAMs via their ramifications. Moreover, RTAMs displayed strong immunostaining for connexin Cx43. Long chains of interconnected RTAMs arose from perivascular clusters and were dispersed within the tumor parenchyma. When expressed, the glucose transporter Glut1 was found in RTAMs and blood vessels, but rarely in cancer cells. ATCs display a very dense network of interconnected RTAMs in direct contact with intermingled cancer cells. To our knowledge this is the first time that such a network is described in a malignant tumor. This network was found in all our studied cases and appeared specific to ATC, since it was not found in differentiated thyroid cancers specimens. Taken together, these results suggest that RTAMs network is directly related to the aggressiveness of the disease via metabolic and trophic functions which remain to be determined.
登录
查看更多内容
影响因子：
6.6
作者：
Bogsrud, Trond Velde;Karantanis, Dimitrios;Lowe, Val J.
通讯作者：
Lowe, Val J.
DOI：
10.1186/1478-811x-7-4
发表时间：
2009-03-12
期刊：
Cell communication and signaling : CCS
影响因子：
--
作者：
Dbouk HA;Mroue RM;El-Sabban ME;Talhouk RS
通讯作者：
Talhouk RS
影响因子：
4.4
作者：
Buhtoiarov, IN;Lum, H;Rakhmilevich, AL
通讯作者：
Rakhmilevich, AL
影响因子：
3.5
作者：
BUSTOS, R;SOBRINO, F
通讯作者：
SOBRINO, F
DOI：
10.1016/j.bbrc.2007.08.066
发表时间：
2007-10-26
影响因子：
3.1
作者：
Giardina, Sarah F.;Mikami, Maya;Yang, Jay
通讯作者：
Yang, Jay