Induced ER chaperones regulate a receptor-like kinase to mediate antiviral innate immune response in plants.
Induced ER chaperones regulate a receptor-like kinase to mediate antiviral innate immune response in plants.
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DOI：
10.1016/j.chom.2009.10.005
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发表时间：
2009-11-19
影响因子：
30.3
通讯作者：
Dinesh-Kumar SP
中科院分区：
文献类型：
--
作者：
Caplan JL;Zhu X;Mamillapalli P;Marathe R;Anandalakshmi R;Dinesh-Kumar SP
植物先天免疫反应需要细胞过程的快速、全局重编程。在这里，我们采用了两种互补的蛋白质组学方法，二维差异凝胶电泳（2D-DIGE）和iTRAQ，以确定差异调节蛋白在防御反应的早期。除了防御相关的蛋白质，上调蛋白质的最大类别的成分是细胞质和内质网（ER）驻留的分子伴侣。ER驻留蛋白二硫键异构酶NbERp 57和NbP 5以及钙网蛋白NbCRT 2和NbCRT 3的沉默导致N免疫受体介导的针对烟草花叶病毒（TMV）的防御的部分丧失。此外，NbCRT 2和NbCRT 3是一种新的诱导受体样激酶（IRK）表达所必需的。IRK是氮介导的过敏反应程序性细胞死亡（HR-PCD）和抗TMV所需的质膜定位蛋白。这些数据支持一个模型，其中ER驻留分子伴侣是先天免疫所必需的膜结合或分泌蛋白的积累所必需的。
The plant innate immune response requires a rapid, global reprogramming of cellular processes. Here we employed two complementary proteomic methods, two-dimensional differential in-gel electrophoresis (2D-DIGE) and iTRAQ, to identify differentially regulated proteins early during a defense response. Besides defense-related proteins, the constituents of the largest category of up-regulated proteins were cytoplasmic- and endoplasmic reticulum (ER)-residing molecular chaperones. Silencing of ER-resident protein disulfide isomerases, NbERp57 and NbP5, and the calreticulins, NbCRT2 and NbCRT3, lead to a partial loss of N immune receptor-mediated defense against Tobacco mosaic virus (TMV). Furthermore, NbCRT2 and NbCRT3 are required for the expression of a novel induced receptor-like kinase (IRK). IRK is a plasma membrane-localized protein required for the N-mediated hypersensitive response programmed cell death (HR-PCD) and resistance to TMV. These data support a model in which ER-resident chaperones are required for the accumulation of membrane bound or secreted proteins that are necessary for innate immunity.
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