Effects of Probiotics Lactobacillus reuteri and Clostridium butyricum on the Expression of Toll-like Receptors, Pro- and Anti-inflammatory Cytokines, and Antimicrobial Peptides in Broiler Chick Intestine.
Effects of Probiotics Lactobacillus reuteri and Clostridium butyricum on the Expression of Toll-like Receptors, Pro- and Anti-inflammatory Cytokines, and Antimicrobial Peptides in Broiler Chick Intestine.
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DOI：
10.2141/jpsa.0190098
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发表时间：
2020-10-25
期刊：
影响因子：
--
通讯作者：
Yoshimura Y
中科院分区：
文献类型：
--
作者：
Terada T;Nii T;Isobe N;Yoshimura Y
本试验旨在研究活益生菌罗伊氏乳杆菌（Lactobacillus reuteri， LR）和丁酸梭菌（Clostridium butyricum， CB）对肉鸡回肠和盲肠先天免疫系统基因表达的影响。雏鸡在孵化后第1 - 6天，每天给予500µl含或不含LR或CB的水。第7天收集回肠和盲肠，采用实时荧光定量PCR技术分析toll样受体（TLRs）、促炎和抗炎细胞因子、抗菌肽（AMPs）的基因表达。小肠回肠TLR2-1表达上调，LR和小肠回肠TLR5表达上调。LR和CB均上调了回肠IL-1β和tgf - β2以及盲肠tgf - β3和tgf - β4的表达。禽β-防御素（AvBD） 1和cathelicidin (CATH) 3在盲肠中表达上调，LR上调AvBD4基因表达。然而，LR可下调回肠中CATH2的表达。这些结果表明，益生菌LR和CB处理通过调节包括tlr、促炎性和抗炎细胞因子以及amp在内的先天免疫分子的表达，影响回肠和盲肠的部分先天防御系统。
The aim of this study was to determine the effects of live probiotics Lactobacillus reuteri (LR) and Clostridium butyricum (CB) on the expression of genes of innate immune system in broiler chick ileum and cecum. Chicks were administered 500 µl water with or without LR or CB, daily from day 1 to 6 after hatching. The ileum and cecum were collected on day 7 for analysis of gene expression of Toll-like receptors (TLRs), pro- and anti-inflammatory cytokines, and antimicrobial peptides (AMPs) using real-time PCR. The expression of TLR2-1 was upregulated by CB in the ileum and that of TLR5 was upregulated by both LR and CB. Expression of IL-1β and TGFβ2 in the ileum and of TGFβ3 and TGFβ4 in the cecum was upregulated by both LR and CB. The gene expressions of avian β-defensin (AvBD) 1 and cathelicidin (CATH) 3 were upregulated by CB and that of AvBD4 was upregulated by LR in the cecum. However, the expression of CATH2 in the ileum was downregulated by LR. These results suggest that probiotic LR and CB treatments affect a part of the innate defense system in the ileum and cecum by modulating the expression of innate immune molecules including TLRs, pro- and anti-inflammatory cytokines, and AMPs.
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影响因子：
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作者：
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DOI：
10.1016/j.ijbiomac.2016.01.031
发表时间：
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