Distinguishing importation from diversification of quinolone-resistant Neisseria gonorrhoeae by molecular evolutionary analysis.
Distinguishing importation from diversification of quinolone-resistant Neisseria gonorrhoeae by molecular evolutionary analysis.
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DOI：
10.1186/1471-2148-7-84
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发表时间：
2007-06-01
影响因子：
3.4
通讯作者：
Viscidi RP
中科院分区：
文献类型：
--
作者：
Pérez-Losada M;Crandall KA;Bash MC;Dan M;Zenilman J;Viscidi RP
区分最近引进的喹诺酮耐药淋球菌进入人口的多样化的耐药菌株已经在人口中是重要的规划有效的感染控制策略。对24株耐喹诺酮类药物的耐药菌株的9个管家基因和gyrA、parC、porB的DNA序列进行了分子进化分析。淋病（QRNG）和2000-2001年期间在以色列收集的24株喹诺酮敏感菌株。系统发育和eBURST分析和分歧时间的估计表明QRNG在3个不同的场合引入，并通过突变，缺失和水平基因转移进行有限的多样化。重建N.淋病人口统计学显示，从1976年到1993年，有效菌株种群规模缓慢下降，1994年到1999年迅速下降，1999年到2001年增加。部分原因是1973年至1994年淋病发病率下降。其他促成因素是选择性地清除抗生素耐药性淋球菌和性工作者传播的增加。1990年代中期的突然下降预示着从1997年至今淋病发病率的上升。自1999年以来，有效菌株群体规模的随后增加反映了淋球菌普查群体的增加和喹诺酮耐药菌株的引入。我们的研究表明，有效地利用种群遗传方法来评估最近和历史的种群动态N。淋病
Distinguishing the recent introduction of quinolone resistant gonococci into a population from diversification of resistant strains already in the population is important for planning effective infection control strategies. We applied molecular evolutionary analyses to DNA sequences from 9 housekeeping genes and gyrA, parC and porB of 24 quinolone resistant N. gonorrhoeae (QRNG) and 24 quinolone sensitive isolates collected in Israel during 2000–2001. Phylogenetic and eBURST analyses and estimates of divergence time indicated QRNG were introduced on 3 separate occasions and underwent limited diversification by mutation, deletion and horizontal gene transfer. Reconstruction of N. gonorrhoeae demography showed a slowly declining effective strain population size from 1976 to 1993, rapid decline between 1994 and 1999, and an increase from 1999 to 2001. This is partially attributable to declining gonorrhea case rates from 1973 to 1994. Additional contributing factors are selective sweeps of antibiotic resistant gonococci and increased transmission from sex workers. The abrupt decline in the mid-1990s heralded an increased incidence of gonorrhea from 1997 to the present. The subsequent increase in effective strain population size since 1999 reflects the increased gonococcal census population and introduction of quinolone resistance strains. Our study demonstrates the effective use of population genetic approaches to assess recent and historical population dynamics of N. gonorrhoeae.
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影响因子：
5.8
作者：
Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
4.9
作者：
Clement, M;Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
3.1
作者：
Dan, Michael;Poch, Francesca;Shohat, Tamy
通讯作者：
Shohat, Tamy
影响因子：
6.4
作者：
Giles, JA;Falconio, J;Bash, MC
通讯作者：
Bash, MC
影响因子：
9.4
作者：
Mavroidi, A;Tzouvelekis, LS;Tzelepi, E
通讯作者：
Tzelepi, E