Real-time monitoring of mono- and dual-species biofilm formation and eradication using microfluidic platform.
Real-time monitoring of mono- and dual-species biofilm formation and eradication using microfluidic platform.
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DOI：
10.1038/s41598-022-13699-9
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发表时间：
2022-06-11
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
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在人类宿主中，细菌金黄色葡萄球菌和真菌白色念珠菌形成混合生物膜，导致严重的死亡和发病率。然而，关于混合生物膜在动态条件下的形成和消除的研究还很缺乏。因此，这项研究使用微流控技术来分析单种和双种(金黄色葡萄球菌和白色念珠菌)生物膜的实时形成，以及使用405 nm激光对已建立的成熟生物膜进行非侵入性光学处理。在被注入微流控芯片上的观察通道之前，人字形混合器将细菌和真菌细胞在生长介质中充分混合。在L培养基流量为1.0微米/分钟作用24小时时，细菌生物被膜覆盖率比真菌生物膜高15%(细菌50%，真菌35%)。另一方面，由于金黄色葡萄球菌和白色念珠菌的集体相互作用，双物种生物膜对 ~ 的覆盖率最高，为96.5%。金黄色葡萄球菌的细胞增殖事件数在12h内高于白色念珠菌，表明金黄色葡萄球菌生物被膜的发育速度快于白色念珠菌。新的原位测试平台显示，405 nm激光1080J/cm2对已建立的金黄色葡萄球菌生物膜有显著的杀菌效果(80%)，而相同的处理方法去除了双物种生物膜中约69%的混合细胞。这项研究表明，所开发的微流控平台可以用于实时监测双物种生物膜的形成和激光对双物种生物膜的抗菌效果。
In a human host, bacterial Staphylococcus aureus and fungal Candida albicans pathogens form a mixed biofilm that causes severe mortality and morbidity. However, research on the formation and eradication of mixed biofilms under dynamic conditions is lacking. Thus, this study employed a microfluidic technique to analyze the real-time formation of mono- and dual-species (S. aureus and C. albicans) biofilms and noninvasive optical treatment of the established mature biofilm using 405-nm laser light. A herringbone mixer thoroughly mixed both bacterial and fungal cells in the growth media before being injected into the observation channels on the microfluidic chip. At a flow rate of 1.0 µL/min of growth media for 24 h, the bacterial biofilm coverage was up to 15% higher than that of the fungal biofilm (50% for bacteria vs. 35% for fungus). On the other hand, the dual-species biofilm yielded the highest coverage of ~ 96.5% because of the collective interaction between S. aureus and C. albicans. The number of cell proliferation events in S. aureus was higher than that of C. albicans for 12 h, which indicates that the S. aureus biofilm was developed faster than C. albicans. The novel in situ test platform showed a significant bactericidal effect (80%) of the 405-nm laser light at 1080 J/cm2 towards the established S. aureus biofilm, whereas the same treatment removed approximately 69% of the mixed cells in the dual-species biofilm. This study revealed that the developed microfluidic platform could be utilized to monitor the formation of dual-species biofilms in real-time and laser-induced antimicrobial effects on dual-species biofilms.
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DOI：
10.3390/pathogens9100784
发表时间：
2020-09-25
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
Lara HH;Lopez-Ribot JL
通讯作者：
Lopez-Ribot JL
影响因子：
2.7
作者：
Hansen, Mads Frederik;Torp, Anders Meyer;Burmolle, Mette
通讯作者：
Burmolle, Mette
DOI：
10.1038/nrmicro2259
发表时间：
2010-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.9
作者：
Harriott, Melphine M.;Noverr, Mairi C.
通讯作者：
Noverr, Mairi C.
DOI：
10.1038/nrmicro.2017.99
发表时间：
2017-12
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Koo H;Allan RN;Howlin RP;Stoodley P;Hall-Stoodley L
通讯作者：
Hall-Stoodley L