Response of Dermal Fibroblasts to Biochemical and Physical Cues in Aligned Polycaprolactone/Silk Fibroin Nanofiber Scaffolds for Application in Tendon Tissue Engineering.
Response of Dermal Fibroblasts to Biochemical and Physical Cues in Aligned Polycaprolactone/Silk Fibroin Nanofiber Scaffolds for Application in Tendon Tissue Engineering.
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皮肤成纤维细胞对对齐的聚辅助酮/丝绸纤维素纳米纤维支架的生化和物理线索的反应，用于在肌腱组织工程中应用。
DOI：
10.3390/nano7080219
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发表时间：
2017-08-11
期刊：
影响因子：
--
通讯作者：
Chen JP
中科院分区：
文献类型：
--
作者：
Chen CH;Chen SH;Kuo CY;Li ML;Chen JP
将丝素蛋白（SF）和纤维排列引入到基于聚己内酯（PCL）的静电纺丝纳米纤维中，作为肌腱组织工程应用的化学和物理线索。无规PCL（RP）纳米纤维，无规PCL/SF（RPSF）纳米纤维和排列的PCL/SF（APSF）纳米纤维的物理化学性能的纤维取向和SF共混效果进行了表征。在纳米纤维上与兔真皮成纤维细胞（RDFB）的体外细胞培养表明SF促进细胞增殖的程度高于纤维排列。扫描电镜观察和细胞骨架染色证实细胞沿纤维轴方向排列。定量实时聚合酶链反应（qRT-PCR）实验表明，上调基因表达的肌腱标记蛋白（I型胶原（Col I），纤连蛋白和双糖蛋白聚糖）的APSF纳米纤维和肌腱重建证实了从Col III基因表达。采用RPSF和APSF支架修复兔跟腱缺损。纤维排列的有益效果从组织学和免疫组织化学染色中得到验证，其中细胞迁移和细胞外基质蛋白沉积倾向于沿APSF纳米纤维的轴向沿着平行方向拉伸，具有增强的Col I和腱生蛋白C产生。生物力学测试表明，细胞种植APSF支架的拉伸刚度和最大载荷分别为正常肌腱值的60.2%和81.3%，这明显高于细胞种植RPSF或脱细胞APSF和RPSF支架。结果表明，APSF支架复合RDFB具有在体修复跟腱差距缺损的潜力，可有效恢复肌腱的功能和结构。
Silk fibroin (SF) and fiber alignment were introduced into polycaprolactone (PCL)-based electrospun nanofibers as chemical and physical cues for tendon tissue engineering applications. The physicochemical properties of random PCL (RP) nanofibers, random PCL/SF (RPSF) nanofibers and aligned PCL/SF (APSF) nanofibers were characterized for fiber orientation and SF blending effects. An in vitro cell culture with rabbit dermal fibroblasts (RDFBs) on nanofibers indicated that SF promotes cell proliferation to a higher extent than fiber alignment. Cells aligned in the direction of fiber axes could be confirmed through scanning electron microscopy (SEM) observation and cytoskeleton staining. The quantitative real-time polymerase chain reaction (qRT-PCR) experiments indicated up-regulated gene expression of tendon marker proteins (type I collagen (Col I), fibronectin and biglycan) on APSF nanofibers and tendon reconstruction was confirmed from Col III gene expression. Animal experiments with Achilles tendon defect repairs in rabbits were carried out with RPSF and APSF scaffolds. The beneficial effects of fiber alignment were verified from histological and immunohistochemical staining, where cell migration and extracellular matrix protein deposition tend to stretch in a parallel direction along the axial direction of APSF nanofibers with enhanced Col I and tenascin C production. Biomechanical testing indicated the tensile stiffness and maximum load of cell-seeded APSF scaffolds were 60.2 and 81.3% of normal tendon values, respectively, which are significantly higher than cell-seeded RPSF or acellular APSF and RPSF scaffolds. These results suggest that APSF nanofiber scaffolds combined with RDFBs have the potential to repair the gap defects of Achilles tendons in vivo and to effectively restore the function and structure of tendons.
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影响因子：
1.9
作者：
Berglund, Maria;Reno, Carol;Wiig, Monica
通讯作者：
Wiig, Monica
影响因子：
--
作者：
Chen, Jingsong;Horan, Rebecca L.;Altman, Gregory H.
通讯作者：
Altman, Gregory H.
影响因子：
5.8
作者：
Chen, Jyh-Ping;Chang, Yin-Shin
通讯作者：
Chang, Yin-Shin
DOI：
10.1034/j.1600-0838.2000.010006312.x
发表时间：
2000-12-01
影响因子：
4.1
作者：
Kannus, P
通讯作者：
Kannus, P
影响因子：
14
作者：
Altman, GH;Diaz, F;Kaplan, DL
通讯作者：
Kaplan, DL