Diet-sourced carbon-based nanoparticles induce lipid alterations in tissues of zebrafish (Danio rerio) with genomic hypermethylation changes in brain.
Diet-sourced carbon-based nanoparticles induce lipid alterations in tissues of zebrafish (Danio rerio) with genomic hypermethylation changes in brain.
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DOI：
10.1093/mutage/gew050
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发表时间：
2017-01
期刊：
影响因子：
2.7
通讯作者：
Martin FL
中科院分区：
文献类型：
--
作者：
Gorrochategui E;Li J;Fullwood NJ;Ying GG;Tian M;Cui L;Shen H;Lacorte S;Tauler R;Martin FL
随着碳基纳米颗粒（CBN）的环境水平不断上升，迫切需要了解其生物效应，以制定适当的风险评估策略。在本文中，我们通过它们的饮食将斑马鱼暴露于四种不同的CBN之一：C60富勒烯（C60），单壁碳纳米管（SWCNT），短多壁碳纳米管（MWCNT）或长MWCNT。采用基于液相色谱-质谱联用（LC-MS）数据文件的“组学”程序，研究了暴露后雄性和雌性斑马鱼三种靶组织（脑、性腺和胃肠道）的脂质变化。选择这些组织是因为它们通常是环境暴露后的靶组织。在所有三种组织中均观察到脂质物质的显著变化。为了进一步探索CBN诱导的脑改变，进行脂质提取物的拉曼显微光谱分析。观察到男性与女性反应不同的脂质变化;此外，似乎也有全球基因组甲基化的一致升高。后一种观察结果在暴露于短MWCNT或SWCNT后的雌性斑马鱼脑组织中最深刻（P < 0.05）。这项研究表明，即使在低水平下，CBN也能够在一系列组织中诱导显著的细胞和基因组修饰。这种改变可能导致对其他影响的敏感性改变，如环境暴露、病理学以及大脑发育改变。
With rising environmental levels of carbon-based nanoparticles (CBNs), there is an urgent need to develop an understanding of their biological effects in order to generate appropriate risk assessment strategies. Herein, we exposed zebrafish via their diet to one of four different CBNs: C60 fullerene (C60), single-walled carbon nanotubes (SWCNT), short multi-walled carbon nanotubes (MWCNTs) or long MWCNTs. Lipid alterations in male and female zebrafish were explored post-exposure in three target tissues (brain, gonads and gastrointestinal tract) using ‘omic’ procedures based in liquid chromatography coupled with mass spectrometry (LC-MS) data files. These tissues were chosen as they are often target tissues following environmental exposure. Marked alterations in lipid species are noted in all three tissues. To further explore CBN-induced brain alterations, Raman microspectroscopy analysis of lipid extracts was conducted. Marked lipid alterations are observed with males responding differently to females; in addition, there also appears to be consistent elevations in global genomic methylation. This latter observation is most profound in female zebrafish brain tissues post-exposure to short MWCNTs or SWCNTs (P < 0.05). This study demonstrates that even at low levels, CBNs are capable of inducing significant cellular and genomic modifications in a range of tissues. Such alterations could result in modified susceptibility to other influences such as environmental exposures, pathology and, in the case of brain, developmental alterations.
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影响因子：
11.4
作者：
Dhawan, Alok;Taurozzi, Julian S.;Tarabara, Volodymyr V.
通讯作者：
Tarabara, Volodymyr V.
影响因子：
11.4
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影响因子：
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作者：
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通讯作者：
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