A SARS-CoV-2 variant with the 12-bp deletion at E gene.
A SARS-CoV-2 variant with the 12-bp deletion at E gene.
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DOI：
10.1080/22221751.2020.1837017
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发表时间：
2020-12
影响因子：
13.2
通讯作者：
Zhu HP
中科院分区：
文献类型：
--
作者：
Sun YS;Xu F;An Q;Chen C;Yang ZN;Lu HJ;Chen JC;Yao PP;Jiang JM;Zhu HP
2019冠状病毒病（COVID-19）大流行仍在持续，并已成为重要的公共卫生威胁。这种疾病是由一种名为严重急性呼吸综合征冠状病毒-2（SARS-CoV-2）的新型冠状病毒感染引起的，到目前为止，对这种病毒的了解甚少。本研究利用噬斑纯化技术，从同一标本中分离纯化了两株SARS-CoV-2病毒株，其中一株命名为F8，E基因缺失12 bp，另一株命名为8X，E基因为野生型。两株病毒的滴度和感染性无明显差异。F_8病毒培养物中S蛋白含量为0.39 μ g/ml，明显高于8X病毒培养物。 用F8株制备的灭活疫苗可引发高水平的IgG滴度和中和抗体滴度，其可持续至少6周，并且在接种后1周和3周分别显著高于8X株。综上所述，我们报道了E基因突变株和野生型SARS-CoV-2株均通过空斑纯化从同一临床样本中分离得到。E基因12 bp的缺失对SARS-CoV-2的复制和免疫原性具有重要意义。
The coronavirus disease 2019 (COVID-19) pandemic is still ongoing and has become an important public health threat. This disease is caused by a new coronavirus named severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection, and so far, little is known about this virus. In this study, by using plaque purification, we purified two SARS-CoV-2 virus strains from the same specimen, one named F8 containing a 12-bp deletion in the E gene and the other named 8X containing the wild-type E gene. There was no significant difference in the viral titer and infectivity of these two strains. The S protein content of the F8 viral culture was 0.39 μg/ml, much higher than that of 8X. An inactivated vaccine made from the F8 strain could trigger high levels of the IgG titer and neutralizing antibody titer, which could last for at least 6 weeks and were significantly higher than those from the 8X strain at 1 and 3 weeks post vaccination, respectively. In conclusion, we reported that both the E gene mutant and wild-type SARS-CoV-2 strains were isolated from the same clinical sample by plaque purification. A 12-bp deletion in the E gene was important for SARS-CoV-2 replication and immunogenicity.
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DOI：
10.1073/pnas.2009637117
发表时间：
2020-06-30
影响因子：
11.1
作者：
Zhang, Renyi;Li, Yixin;Molina, Mario J.
通讯作者：
Molina, Mario J.
影响因子：
4.6
作者：
Sun YS;Li YJ;Xia Y;Xu F;Wang WW;Yang ZN;Lu HJ;Chen ZP;Miao ZP;Liang WF;Xu ZY;Dong HJ;Qiu DH;Zhu ZY;van der Veen S;Qian J;Zhou B;Yao PP;Zhu HP
通讯作者：
Zhu HP
影响因子：
6.4
作者：
Castaño-Rodriguez C;Honrubia JM;Gutiérrez-Álvarez J;DeDiego ML;Nieto-Torres JL;Jimenez-Guardeño JM;Regla-Nava JA;Fernandez-Delgado R;Verdia-Báguena C;Queralt-Martín M;Kochan G;Perlman S;Aguilella VM;Sola I;Enjuanes L
通讯作者：
Enjuanes L
影响因子：
64.5
作者：
Korber, Bette;Fischer, Will M.;Montefiori, David C.
通讯作者：
Montefiori, David C.
影响因子：
5.4
作者：
Lamirande, Elaine W.;DeDiego, Marta L.;Subbarao, Kanta
通讯作者：
Subbarao, Kanta