Distribution and identity of the earliest proliferating progeny of colony-forming cells in regenerating murine spleen and bone marrow.

Distribution and identity of the earliest proliferating progeny of colony-forming cells in regenerating murine spleen and bone marrow.
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再生小鼠脾脏和骨髓中集落形成细胞最早增殖后代的分布和身份。

DOI:
10.1002/aja.1001630203
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发表时间:
1982
期刊:
The American journal of anatomy
影响因子:
--
通讯作者:
Rosse,C
Rosse,C
中科院分区:
--
文献类型:
--
作者:
Wolf,N;Rosse,C

文献摘要

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本文对小鼠脾脏极早期造血细胞集落的发育部位和细胞类型进行了研究。移植后2、3和4天,在处死前12、6和0.5小时注射3 H-TdR的超致死剂量照射受体小鼠的脾切片放射自显影照片以及脾和骨髓涂片上鉴定移植骨髓细胞的增殖后代。令人惊讶的是,未移植、照射小鼠的脾脏在白色髓中含有增殖的中型和大型淋巴细胞,这些淋巴细胞的数量在观察期间增加。移植细胞的早期后代可以清楚地从标记的土著细胞中区分出来,因为它们在脾被膜下或红髓的小静脉和小梁附近形成离散的结节或集落。这些细胞在第2天被鉴定为移行细胞或未知的造血母细胞,在第4天包括早期红系细胞和小淋巴细胞。有证据表明3 H-TdR标记的细胞通过脾窦运输。
A study was made of the sites of development and the types of cells found in very early hemopietic colomes in the mouse spleen. Two, 3, and 4 days after transplantation, the proliferating descendants of transplanted bone‐marrow cells were identified on radioautographs of spleen sections and on spleen and bone‐marrow smears of supralethally irradiated recipient mice which were injected with3H‐TdR at 12, 6, and 0.5 hours before sacrifice. Surprisingly the spleens of nontransplanted, irradiated mice contained proliferating medium and large lymphocytes in the white pulp which increased in numbers during the observation period. The early descendants of transplanted cells that lodged in the spleen could be clearly distinguished from the labeled indigenous cells because they formed discrete nodules or colonies beneath the splenic capsule or in the vicinity of venules and trabeculae of the red pulp. These cells were identifiable on day 2 as transitional cells or unknown hemopoietic blasts and on day 4 included early erythroid cells and small lymphocytes. There was evidence for the traffic of3H‐TdR‐labeled cells through the splenic sinusoids.
DOI: 10.1111/j.1365-2184.1974.tb00402.x
发表时间: 1974
期刊: Cell Proliferation
影响因子: 8.5
作者:
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发表时间: 1974
影响因子: 2.6
作者:
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从大鼠骨髓中分离琼脂集落形成细胞 1
DOI: 10.3181/00379727-145-37843
发表时间: 1974
影响因子: --
作者:
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发表时间: 1972
期刊: Cell Proliferation
影响因子: 8.5
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DOI: --
发表时间: 1978
期刊: Blood cells
影响因子: --
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