Identification and characterization of mushroom body neurons that regulate fat storage in Drosophila.
Identification and characterization of mushroom body neurons that regulate fat storage in Drosophila.
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DOI：
10.1186/s13064-018-0116-7
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发表时间：
2018-08-13
影响因子：
3.6
通讯作者：
Zinn K
中科院分区：
文献类型：
--
作者：
Al-Anzi B;Zinn K
在早期的一项研究中，我们确定了两个神经元群体，C673a和Fru-GAL4，它们调节果蝇的脂肪储存。这两个种群与昆虫大脑中被称为蘑菇体(MB)的结构部分重叠，蘑菇体在记忆形成中起着关键作用。这种重叠促使我们检查MB是否也参与了脂肪储存的动态平衡。使用各种转基因试剂，我们有选择地操纵MB和相关神经元不同部分的神经活动，以破译它们在脂肪储存调节中的作用。我们的数据显示，沉默投射到α‘β脑叶的MB神经元减少了新生脂肪酸的合成并导致瘦削，而相同神经元的持续过度激活则会导致过量进食并产生肥胖。α‘β神经元对抗并支配C673a和FruGal4神经元的脂肪调节功能。我们还表明，投射到γ叶的MB神经元也调节脂肪储存，可能是因为它们是Fru神经元的子集。我们能够识别其活动影响脂肪储存、摄食和新陈代谢的输入和输出神经元。支配β‘2室的胆碱能输出神经元(MBon-β’2MP和MBon-γ5β‘2a)调节食物的消耗，而支配α’室的谷氨酸能输出神经元(MBon-γ2α‘1和MBon-α’2)控制脂肪代谢。我们发现了一个新的脂肪储存调节中心，即MB的α‘β叶。我们还描绘了参与α‘β脑叶活动的神经元回路，并表明食物摄取和脂肪代谢由不同的突触后神经元控制，这些突触后神经元被分离到不同的输出路径。本文的在线版本(10.1186/s13064-0180116-7)包含补充材料，可供授权用户使用。
In an earlier study, we identified two neuronal populations, c673a and Fru-GAL4, that regulate fat storage in fruit flies. Both populations partially overlap with a structure in the insect brain known as the mushroom body (MB), which plays a critical role in memory formation. This overlap prompted us to examine whether the MB is also involved in fat storage homeostasis. Using a variety of transgenic agents, we selectively manipulated the neural activity of different portions of the MB and associated neurons to decipher their roles in fat storage regulation. Our data show that silencing of MB neurons that project into the α’β’ lobes decreases de novo fatty acid synthesis and causes leanness, while sustained hyperactivation of the same neurons causes overfeeding and produces obesity. The α’β’ neurons oppose and dominate the fat regulating functions of the c673a and Fru-GAL4 neurons. We also show that MB neurons that project into the γ lobe also regulate fat storage, probably because they are a subset of the Fru neurons. We were able to identify input and output neurons whose activity affects fat storage, feeding, and metabolism. The activity of cholinergic output neurons that innervating the β’2 compartment (MBON-β’2mp and MBON-γ5β’2a) regulates food consumption, while glutamatergic output neurons innervating α’ compartments (MBON-γ2α’1 and MBON-α’2) control fat metabolism. We identified a new fat storage regulating center, the α’β’ lobes of the MB. We also delineated the neuronal circuits involved in the actions of the α’β’ lobes, and showed that food intake and fat metabolism are controlled by separate sets of postsynaptic neurons that are segregated into different output pathways. The online version of this article (10.1186/s13064-018-0116-7) contains supplementary material, which is available to authorized users.
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DOI：
10.1073/pnas.0702726104
发表时间：
2007-05-15
影响因子：
11.1
作者：
Ja, William W.;Carvalho, Gil B.;Benzer, Seymour
通讯作者：
Benzer, Seymour
苍蝇和蠕虫中脂肪储存的遗传学：出了什么问题？
影响因子：
3.7
作者：
Al-Anzi B;Zinn K
通讯作者：
Zinn K
影响因子：
7.7
作者：
Aso Y;Hattori D;Yu Y;Johnston RM;Iyer NA;Ngo TT;Dionne H;Abbott LF;Axel R;Tanimoto H;Rubin GM
通讯作者：
Rubin GM
影响因子：
16.2
作者：
Al-Anzi, Bader;Sapin, Viveca;Waters, Christopher;Zinn, Kai;Wyman, Robert J.;Benzer, Seymour
通讯作者：
Benzer, Seymour
影响因子：
8.8
作者：
Jenett, Arnim;Rubin, Gerald M.;Zugates, Christopher T.
通讯作者：
Zugates, Christopher T.