A sleep-like state in Hydra unravels conserved sleep mechanisms during the evolutionary development of the central nervous system.
A sleep-like state in Hydra unravels conserved sleep mechanisms during the evolutionary development of the central nervous system.
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DOI：
10.1126/sciadv.abb9415
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发表时间：
2020-10
期刊：
影响因子：
13.6
通讯作者：
Itoh TQ
中科院分区：
文献类型：
--
作者：
Kanaya HJ;Park S;Kim JH;Kusumi J;Krenenou S;Sawatari E;Sato A;Lee J;Bang H;Kobayakawa Y;Lim C;Itoh TQ
没有大脑的淡水水龙通过在动物物种中保存或重新编程的机制来控制睡眠-觉醒周期。甚至在线虫和节肢动物中也观察到了睡眠行为，但人们对睡眠调节机制是如何在进化过程中出现的知之甚少。在这里，我们报告了一种具有原始神经组织的刺蛇的睡眠状态。九头蛇睡眠是由动态平衡塑造的，是细胞增殖所必需的，但它缺乏自由运行的昼夜节律。相反，我们检测到了可能由九头蛇睡眠背后的超级振荡器产生的4小时节律。对睡眠剥夺的九头蛇进行的基因芯片分析显示，212个基因的表达与睡眠有关，包括cGMP依赖的蛋白激酶1(PRKG1)和鸟氨酸转氨酶。褪黑激素、GABA和PRKG1的促进睡眠作用在九头蛇中是保守的。然而，唤醒多巴胺出人意料地诱导了九头蛇的睡眠。在九头蛇和果蝇中，鸟氨酸代谢对睡眠的相反影响也很明显，这表明它们的睡眠调节功能发生了进化上的切换。因此，睡眠相关的生理和睡眠调节成分可能已经在分子水平上在无大脑的后生动物门中获得并相应地重新编程。
The brain-less freshwater polyp Hydra controls sleep-wake cycles by mechanisms conserved or reprogrammed among animal species. Sleep behaviors are observed even in nematodes and arthropods, yet little is known about how sleep-regulatory mechanisms have emerged during evolution. Here, we report a sleep-like state in the cnidarian Hydra vulgaris with a primitive nervous organization. Hydra sleep was shaped by homeostasis and necessary for cell proliferation, but it lacked free-running circadian rhythms. Instead, we detected 4-hour rhythms that might be generated by ultradian oscillators underlying Hydra sleep. Microarray analysis in sleep-deprived Hydra revealed sleep-dependent expression of 212 genes, including cGMP-dependent protein kinase 1 (PRKG1) and ornithine aminotransferase. Sleep-promoting effects of melatonin, GABA, and PRKG1 were conserved in Hydra. However, arousing dopamine unexpectedly induced Hydra sleep. Opposing effects of ornithine metabolism on sleep were also evident between Hydra and Drosophila, suggesting the evolutionary switch of their sleep-regulatory functions. Thus, sleep-relevant physiology and sleep-regulatory components may have already been acquired at molecular levels in a brain-less metazoan phylum and reprogrammed accordingly.
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影响因子：
15.9
作者：
Bosch TCG;Klimovich A;Domazet-Lošo T;Gründer S;Holstein TW;Jékely G;Miller DJ;Murillo-Rincon AP;Rentzsch F;Richards GS;Schröder K;Technau U;Yuste R
通讯作者：
Yuste R
影响因子：
14.9
作者：
Kriventseva EV;Kuznetsov D;Tegenfeldt F;Manni M;Dias R;Simão FA;Zdobnov EM
通讯作者：
Zdobnov EM
影响因子：
1.6
作者：
Garm, A.;Bielecki, J.;Nilsson, D. -E.
通讯作者：
Nilsson, D. -E.
DOI：
10.1016/j.cub.2010.05.029
发表时间：
2010-07-13
期刊：
Current biology : CB
影响因子：
--
作者：
Keene AC;Duboué ER;McDonald DM;Dus M;Suh GS;Waddell S;Blau J
通讯作者：
Blau J
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro