Clock and cycle limit starvation-induced sleep loss in Drosophila.
Clock and cycle limit starvation-induced sleep loss in Drosophila.
复制标题
DOI：
10.1016/j.cub.2010.05.029
复制
发表时间：
2010-07-13
期刊：
影响因子：
--
通讯作者：
Blau J
中科院分区：
文献类型：
--
作者：
Keene AC;Duboué ER;McDonald DM;Dus M;Suh GS;Waddell S;Blau J
哺乳动物控制睡眠和进食等重要功能的神经系统是紧密相连的：睡眠不足会促进进食，而饥饿会抑制睡眠。在这里，我们发现果蝇的饥饿会有效抑制睡眠，这表明这两种稳态调节行为也整合在果蝇中。饥饿的睡眠抑制作用与蘑菇体无关，蘑菇体是果蝇大脑中先前确定的睡眠场所，因此受到不同的神经回路的调节。生物钟基因 Clock (Clk) 和 Cycle (cyc) 对于饥饿期间适当的睡眠抑制至关重要。然而，睡眠抑制与光提示和昼夜节律无关，因为饥饿期突变体像野生型果蝇一样睡觉。通过选择性地针对表达 Clk 的神经元亚群，我们将观察到的睡眠表型定位于位于背侧的昼夜节律神经元。这些发现表明，Clk 和 cyc 在饥饿期间发挥作用，调节果蝇是否睡觉或寻找食物的冲突。
Neural systems controlling the vital functions of sleep and feeding in mammals are tightly inter-connected: sleep deprivation promotes feeding, while starvation suppresses sleep. Here we show that starvation in Drosophila potently suppresses sleep suggesting that these two homeostatically regulated behaviors are also integrated in flies. The sleep suppressing effect of starvation is independent of the mushroom bodies, a previously identified sleep locus in the fly brain, and therefore is regulated by distinct neural circuitry. The circadian clock genes Clock (Clk) and cycle (cyc) are critical for proper sleep suppression during starvation. However, the sleep suppression is independent of light cues and of circadian rhythms because starved period mutants sleep like wild type flies. By selectively targeting subpopulations of Clk-expressing neurons we localize the observed sleep phenotype to the dorsally located circadian neurons. These findings show that Clk and cyc act during starvation to modulate the conflict of whether flies sleep or search for food.
登录
查看更多内容
DOI：
10.1016/j.cub.2009.12.055
发表时间：
2010-02-23
期刊：
Current biology : CB
影响因子：
--
作者：
Chatterjee A;Tanoue S;Houl JH;Hardin PE
通讯作者：
Hardin PE
影响因子：
3.5
作者：
Hendricks, JC;Lu, SM;Sehgal, A
通讯作者：
Sehgal, A
影响因子：
11.4
作者：
Allada, R;Kadener, S;Rosbash, M
通讯作者：
Rosbash, M
影响因子：
3.5
作者：
Mattaliano, Mark D.;Montana, Enrico S.;Griffith, Leslie C.
通讯作者：
Griffith, Leslie C.
影响因子：
56.9
作者：
Osborne, KA;Robichon, A;Sokolowski, MB
通讯作者：
Sokolowski, MB