Molecular characterization of individual D3 dopamine receptor-expressing cells isolated from multiple brain regions of a novel mouse model.
Molecular characterization of individual D3 dopamine receptor-expressing cells isolated from multiple brain regions of a novel mouse model.
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DOI：
10.1007/s00429-012-0383-8
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发表时间：
2012-10
影响因子：
3.1
通讯作者：
Kuzhikandathil, Eldo V.
中科院分区：
文献类型：
--
作者：
Li, Ying;Kuzhikandathil, Eldo V.
在多巴胺受体中，D3受体亚型的表达和功能尚不清楚。该受体对多巴胺和许多靶向多巴胺受体的药物具有最高的亲和力。在本文中，我们在单细胞水平上研究了从35或70日龄的雄性和雌性小鼠的不同脑区分离的D3受体表达细胞的特征。脑区包括伏隔核、胼胝体岛、嗅结节、脾后皮质、背下带、乳状体、杏仁核和隔膜。在drd3增强绿色荧光蛋白转基因小鼠中进行了表达分析，这些小鼠报告了D3受体mRNA的内源性表达。利用单细胞逆转录酶PCR，我们确定了这些小鼠中表达D3受体的荧光细胞是神经元还是胶质细胞，以及它们是谷氨酸能、gaba能还是儿茶酚胺能。接下来，我们确定荧光细胞是否共表达其他四种多巴胺受体亚型、腺苷酸环化酶V （ACV）亚型和G蛋白偶联内向整流钾（GIRK）通道的三种不同亚型。结果表明，D3受体在神经元中表达，并在谷氨酸能和氨基丁酸能神经元中有区域特异性表达。D3受体主要与D1和D2多巴胺受体共表达，在共表达模式上存在区域、性别和年龄依赖性差异。共表达D3受体和ACV或GIRK通道的细胞百分比因脑区、性别和年龄的不同而有显著差异。本文报道的小鼠脑D3受体表达细胞的分子表征将有助于D3受体在生理和病理生理上的功能表征。
Among dopamine receptors, the expression and function of the D3 receptor subtype is not well understood. The receptor has the highest affinity for dopamine and many drugs that target dopamine receptors. In this paper, we examined, at the single cell level, the characteristics of D3 receptor-expressing cells isolated from different brain regions of male and female mice that were either 35 or 70 days old. The brain regions included nucleus accumbens, Islands of Calleja, olfactory tubercle, retrosplenial cortex, dorsal subiculum, mammillary body, amygdala and septum. The expression analysis was done in the drd3-enhanced green fluorescent protein transgenic mice that report the endogenous expression of D3 receptor mRNA. Using single cell reverse transcriptase PCR, we determined if the D3 receptor-expressing fluorescent cells in these mice were neurons or glia and if they were glutamatergic, GABAergic or catecholaminergic. Next, we determined if the fluorescent cells co-expressed the four other dopamine receptor subtypes, adenylate cyclase V (ACV) isoform, and three different isoforms of G protein-coupled inward rectifier potassium (GIRK) channels. The results suggest that D3 receptor is expressed in neurons, with region-specific expression in glutamatergic and GABAergic neurons. The D3 receptor primarily co-expressed with D1 and D2 dopamine receptors with regional, sex and age-dependent differences in the co-expression pattern. The percentage of cells co-expressing D3 receptor and ACV or GIRK channels varied significantly by brain region, sex and age. The molecular characterization of D3 receptor-expressing cells in mouse brain reported here will facilitate the characterization of D3 receptor function in physiology and pathophysiology.
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影响因子：
4.7
作者：
Kim Y;Wang WZ;Comte I;Pastrana E;Tran PB;Brown J;Miller RJ;Doetsch F;Molnár Z;Szele FG
通讯作者：
Szele FG
影响因子：
16.2
作者：
Fanselow, Michael S.;Dong, Hong-Wei
通讯作者：
Dong, Hong-Wei
影响因子：
5.7
作者：
Koshibu, K;Levitt, P;Ahrens, ET
通讯作者：
Ahrens, ET
影响因子：
1.7
作者：
Andersen, SL;Rutstein, M;Teicher, MH
通讯作者：
Teicher, MH
DOI：
10.1523/jneurosci.2107-11.2011
发表时间：
2011-08-10
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Corcoran KA;Donnan MD;Tronson NC;Guzmán YF;Gao C;Jovasevic V;Guedea AL;Radulovic J
通讯作者：
Radulovic J