Assessing motile cilia coverage and beat frequency in mammalian in vitro cell culture tissues.
Assessing motile cilia coverage and beat frequency in mammalian in vitro cell culture tissues.
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DOI：
10.1098/rsos.230185
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发表时间：
2023-08
影响因子：
3.5
通讯作者：
Cicuta, Pietro
中科院分区：
文献类型：
--
作者：
Fradique, Ricardo;Causa, Erika;Delahousse, Clara;Kotar, Jurij;Pinte, Laetitia;Vallier, Ludovic;Vila-Gonzalez, Marta;Cicuta, Pietro
纤毛密度、分布和搏动频率是呼吸道上皮组织的重要特性。这些参数对于诊断原发睫状肌运动障碍和检验体外模型至关重要，包括那些来自诱导多能干细胞的模型。视频显微镜可以用来表征这些参数，但目前可用的大多数工具提供的信息类型有限，通常只描述非常小区域的纤毛搏动频率，而需要人工干预和培训才能使用。我们提出了一种新的、开源的方法，可以在无需用户干预的情况下，以自动的方式全面表征纤毛跳动频率和运动纤毛复盖率。我们展示了区分不同覆盖密度的能力，在更大的视野中识别即使是小块的纤毛，并充分表征所有移动区域的纤毛跳动频率。我们还表明，该方法可以用于组合多个视场以更好地描述样本，而不依赖于预先选择的小的感兴趣区域。这是通过一个简单的文件处理图形用户界面发布的，能够在典型的个人计算机上在几分钟内对各个视野进行全面分析。
Cilia density, distribution and beating frequency are important properties of airway epithelial tissues. These parameters are critical in diagnosing primary ciliary dyskinesia and examining in vitro models, including those derived from induced pluripotent stem cells. Video microscopy can be used to characterize these parameters, but most tools available at the moment are limited in the type of information they can provide, usually only describing the ciliary beat frequency of very small areas, while requiring human intervention and training for their use. We propose a novel and open-source method to fully characterize cilia beating frequency and motile cilia coverage in an automated fashion without user intervention. We demonstrate the ability to differentiate between different coverage densities, identifying even small patches of cilia in a larger field of view, and to fully characterize the cilia beating frequency of all moving areas. We also show that the method can be used to combine multiple fields of view to better describe a sample without relying on small pre-selected regions of interest. This is released with a simple graphical user interface for file handling, enabling a full analysis of individual fields of view in a few minutes on a typical personal computer.
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影响因子：
3.4
作者：
Feriani, Luigi;Juenet, Maya;Cicuta, Pietro
通讯作者：
Cicuta, Pietro
DOI：
10.1165/rcmb.2011-0031oc
发表时间：
2011-11-01
影响因子：
6.4
作者：
Hackett, Tillie-Louise;Singhera, Gurpreet K.;Knight, Darryl A.
通讯作者：
Knight, Darryl A.
影响因子：
4.6
作者：
Sampaio, Pedro;da Silva, Monica Ferro;Lopes, Susana S.
通讯作者：
Lopes, Susana S.
DOI：
10.1046/j.1365-2818.2003.01209.x
发表时间：
2003-08-01
期刊：
JOURNAL OF MICROSCOPY-OXFORD
影响因子：
--
作者：
Sisson, JH;Stoner, JA;Wyatt, TA
通讯作者：
Wyatt, TA
影响因子：
10.6
作者：
Choi, Woo June;Yoon, Jung-Ki;Kim, Jun Ki
通讯作者：
Kim, Jun Ki