Ivermectin inhibits the sporogony of Plasmodium falciparum in Anopheles gambiae.
Ivermectin inhibits the sporogony of Plasmodium falciparum in Anopheles gambiae.
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DOI：
10.1186/1475-2875-11-381
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发表时间：
2012-11-21
期刊：
影响因子：
3
通讯作者：
Richardson JH
中科院分区：
文献类型：
--
作者：
Kobylinski KC;Foy BD;Richardson JH
在实验室和现场，伊维菌素在血餐中摄入后可降低冈比亚按蚊的存活率。此外，伊维菌素大规模药物管理在塞内加尔已被证明，以减少比例的恶性疟原虫子孢子，含有安。冈比亚。本研究探讨了伊维菌素是否能抑制恶性疟原虫的孢子繁殖。冈比亚。冈比亚按蚊G3菌株以交错间隔在人血餐中饲喂两种浓度的伊维菌素（LC 25和LC 5）沿着恶性疟原虫NF 54。蚊子与寄生虫同时摄入伊维菌素（DPI 0），或在寄生虫摄入后3天（DPI 3）、6天（DPI 6）和9天（DPI 9），或寄生虫摄入前3天（DPI-3）。在寄生虫摄入后7、12或14天解剖蚊子，记录卵囊或子孢子的流行率。为了确定是否含有恶性疟原虫子孢子。冈比亚比未感染的对照组对伊维菌素更敏感，记录了在DPI 0摄入恶性疟原虫或对照血粉，然后在DPI 14摄入含有伊维菌素（LC 25）的第二次血粉的蚊子的存活率。伊维菌素（LC 25）与寄生虫共食（DPI 0）可降低寄生虫的比例。冈比亚按蚊卵囊和子孢子的发育率分别为15.4842和19.9643（χ2 = 0.0002，P < 0.0001）。伊维菌素（LC 25）可降低DPI 6（χ2 = 8.5103，P = 0.0044）和DPI 9（χ2 = 14.7998，P < 0.0001）时血清中的AN比例。冈比亚按蚊在DPI 3组中未出现子孢子（χ2 = 0.0113，P = 1）。伊维菌素（LC_5）与寄生虫共食（DPI_0）不能降低寄生虫的比例。冈比亚的卵囊（χ2 = 4.2518，P = 0.0577）或子孢子（χ2 = 2.3636，P = 0.1540），然而，当摄入DPI-3时，An.冈比亚产子孢子率降低（χ2 = 8.4806，P = 0.0047）。恶性疟原虫感染显著降低了AN的存活率。与摄入不含寄生虫的初级血粉的对照蚊子相比，在DPI 14时摄入伊维菌素（LC 25）的冈比亚蚊子（χ2 = 4.97，P = 0.0257）。亚致死浓度的伊维菌素可抑制恶性疟原虫的孢子繁殖。冈比亚。这些发现支持伊维菌素用于控制恶性疟原虫传播的效用。
When ingested in a blood meal, ivermectin has been shown to reduce the survivorship of Anopheles gambiae in the laboratory and field. Furthermore, ivermectin mass drug administrations in Senegal have been shown to reduce the proportion of Plasmodium falciparum-sporozoite-containing An. gambiae. This study addresses whether ivermectin inhibits sporogony of P. falciparum in An. gambiae. Anophele gambiae s.s. G3 strain were fed two concentrations of ivermectin (LC25 and LC5) along with P. falciparum NF54 in human blood meals at staggered intervals. Mosquitoes ingested ivermectin concurrent with parasites (DPI 0), or at three (DPI 3), six (DPI 6), and nine (DPI 9) days post parasite ingestion, or three days prior (DPI −3) to parasite ingestion. Mosquitoes were dissected at seven, twelve or fourteen days post parasite ingestion and either oocyst or sporozoite prevalence was recorded. To determine if P. falciparum sporozoite-containing An. gambiae were more susceptible to ivermectin than uninfected controls, survivorship was recorded for mosquitoes which ingested P. falciparum or control blood meal on DPI 0 and then a second blood meal containing ivermectin (LC25) on DPI 14. Ivermectin (LC25) co-ingested (DPI 0) with parasites reduced the proportion of An. gambiae that developed oocysts (χ2 = 15.4842, P = 0.0002) and sporozoites (χ2 = 19.9643, P < 0.0001). Ivermectin (LC25) ingested DPI 6 (χ2 = 8.5103, P = 0.0044) and 9 (χ2 = 14.7998, P < 0.0001) reduced the proportion of An. gambiae that developed sporozoites but not when ingested DPI 3 (χ2 = 0.0113, P = 1). Ivermectin (LC5) co-ingested (DPI 0) with parasites did not reduce the proportion of An. gambiae that developed oocysts (χ2 = 4.2518, P = 0.0577) or sporozoites (χ2 = 2.3636, P = 0.1540), however, when ingested DPI −3 the proportion of An. gambiae that developed sporozoites was reduced (χ2 = 8.4806, P = 0.0047). Plasmodium falciparum infection significantly reduced the survivorship of An. gambiae that ingested ivermectin (LC25) on DPI 14 compared to control mosquitoes that ingested a primary blood meal without parasites (χ2 = 4.97, P = 0.0257). Ivermectin at sub-lethal concentrations inhibits the sporogony of P. falciparum in An. gambiae. These findings support the utility of ivermectin for P. falciparum transmission control.
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影响因子：
64.8
作者：
CULLY, DF;VASSILATIS, DK;ARENA, JP
通讯作者：
ARENA, JP
DOI：
10.1179/000349809x12459740922138
发表时间：
2009-09-01
影响因子：
--
作者：
Fritz, M. L.;Siegert, P. Y.;Miller, J. R.
通讯作者：
Miller, J. R.
影响因子：
9.6
作者：
Ferguson, HM;Read, AF
通讯作者：
Read, AF
影响因子：
2.7
作者：
Butters, Matthew P.;Kobylinski, Kevin C.;Foy, Brian D.
通讯作者：
Foy, Brian D.
影响因子：
3
作者：
Bousema, Teun;Okell, Lucy;Drakeley, Chris
通讯作者：
Drakeley, Chris