Cloned multipotential precursors from the mouse cerebrum require FGF-2, whereas glial restricted precursors are stimulated with either FGF-2 or EGF
Cloned multipotential precursors from the mouse cerebrum require FGF-2, whereas glial restricted precursors are stimulated with either FGF-2 or EGF
复制标题
来自小鼠大脑的克隆多能前体细胞需要 FGF-2，而神经胶质细胞限制性前体细胞则需要 FGF-2 或 EGF 刺激
作者：
T. Kilpatrick;P. Bartlett
已报道成纤维细胞生长因子-2（FGF-2）和表皮生长因子（EGF）均刺激发育中的CNS中的前体。为了澄清这些影响，我们使用克隆分析，以确定与每个因素刺激的前体细胞的谱系潜力，在两个发展阶段。发现在从胚胎第17天（E17）小鼠的大脑分离的细胞中，FGF-2刺激多能前体（其产生神经元和星形胶质细胞）和定向神经胶质前体。相反，EGF仅刺激胶质限制性前体。因此，看来，多能细胞，以前确定为目前在E10，保持选择性响应FGF-2，并在E17观察到的EGF反应反映了一个新的限制类前体的存在，而不是开关的多能细胞的因子特异性。
Fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF) have both been reported to stimulate precursors in the developing CNS. To clarify these effects, we used clonal analysis to determine the lineage potential of precursors stimulated with each factor, at two stages of development. It was found that in cells isolated from the cerebrum of embryonic day 17 (E17) mice, FGF-2 stimulated both a multipotential precursor, which gave rise to neurons and astrocytes, and a committed glial precursor. In contrast, EGF only stimulated the glial restricted precursor. Thus, it appears that the multipotential cell, previously identified to be present at E10, remains selectively responsive to FGF-2, and that the EGF responsiveness observed at E17 reflects the presence of a new restricted class of precursors, rather than a switch in factor specificity of the multipotential cell.
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影响因子：
56.9
作者：
WALSH, C;CEPKO, CL
通讯作者：
CEPKO, CL
影响因子：
4.6
作者：
Larry S. Sherman;K. Stocker;Richard S. Morrison;G. Ciment
通讯作者：
Larry S. Sherman;K. Stocker;Richard S. Morrison;G. Ciment
DOI：
10.1073/pnas.90.5.2074
发表时间：
1993-03-01
影响因子：
11.1
作者：
LOIS, C;ALVAREZBUYLLA, A
通讯作者：
ALVAREZBUYLLA, A
DOI：
10.1073/pnas.90.8.3602
发表时间：
1993-04-15
影响因子：
11.1
作者：
RAY, J;PETERSON, DA;GAGE, FH
通讯作者：
GAGE, FH