Shedding light on the microbial community of the macropod foregut using 454-amplicon pyrosequencing.
Shedding light on the microbial community of the macropod foregut using 454-amplicon pyrosequencing.
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DOI：
10.1371/journal.pone.0061463
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Klieve AV
中科院分区：
文献类型：
--
作者：
Gulino LM;Ouwerkerk D;Kang AY;Maguire AJ;Kienzle M;Klieve AV
20个来自澳大利亚昆士兰州的5个地点，放牧的各种原生牧场的macropod进行了调查和细菌群落的前肠进行了检查，使用454-扩增子焦磷酸测序。具体地，检查16 S rRNA基因的V3/V4区。在数据集中识别出总共5040个OTU（过滤后）。32个OTU被鉴定为属于厚壁菌门或拟杆菌门（梭菌目/拟杆菌目）的“共有”OTUS（即存在于所有样品中）。这些门占主导地位的一般微生物群落在所有的macropods。在共享OTU内代表的属包括：未分类的瘤胃球菌科、未分类的毛螺菌科、未分类的梭菌目、消化球菌属、粪球菌属、链球菌属、布劳特氏菌，Ruminoccocus sp.，真杆菌属，Dorea sp.，颤螺旋菌属和丁酸弧菌属。每个宿主物种（Macropus rufus，Macropus luteus和Macropus robustus）的前肠样品的细菌群落的组成是显着不同的允许宿主物种之间的差异的基础上α和β多样性措施。具体地，鉴定了分离三种宿主物种的11个优势OTU，并将其分类为：未分类的瘤胃球菌科、未分类的拟杆菌目、普雷沃氏菌属。和一种变形蔗糖共生球菌在样品中还鉴定了推定的还原产乙酸菌和纤维分解菌。未来的工作将调查在这些生态系统中的纤维溶解和产乙酸的存在和作用。理想情况下，这些生物体的分离和表征将用于提高牛的饲料效率，减少甲烷，并可能用于其他行业，如生物燃料行业。
Twenty macropods from five locations in Queensland, Australia, grazing on a variety of native pastures were surveyed and the bacterial community of the foregut was examined using 454-amplicon pyrosequencing. Specifically, the V3/V4 region of 16S rRNA gene was examined. A total of 5040 OTUs were identified in the data set (post filtering). Thirty-two OTUs were identified as ‘shared’ OTUS (i.e. present in all samples) belonging to either Firmicutes or Bacteroidetes (Clostridiales/Bacteroidales). These phyla predominated the general microbial community in all macropods. Genera represented within the shared OTUs included: unclassified Ruminococcaceae, unclassified Lachnospiraceae, unclassified Clostridiales, Peptococcus sp. Coprococcus spp., Streptococcus spp., Blautia sp., Ruminoccocus sp., Eubacterium sp., Dorea sp., Oscillospira sp. and Butyrivibrio sp. The composition of the bacterial community of the foregut samples of each the host species (Macropus rufus, Macropus giganteus and Macropus robustus) was significantly different allowing differentiation between the host species based on alpha and beta diversity measures. Specifically, eleven dominant OTUs that separated the three host species were identified and classified as: unclassified Ruminococcaceae, unclassified Bacteroidales, Prevotella spp. and a Syntrophococcus sucromutans. Putative reductive acetogens and fibrolytic bacteria were also identified in samples. Future work will investigate the presence and role of fibrolytics and acetogens in these ecosystems. Ideally, the isolation and characterization of these organisms will be used for enhanced feed efficiency in cattle, methane mitigation and potentially for other industries such as the biofuel industry.
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影响因子：
1
作者：
Beauchemin, KA;Rode, LM;Sewalt, VJH
通讯作者：
Sewalt, VJH
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
3.7
作者：
Jami E;Mizrahi I
通讯作者：
Mizrahi I
影响因子：
4.4
作者：
HESPELL, RB;WOLF, R;BOTHAST, RJ
通讯作者：
BOTHAST, RJ
DOI：
10.1099/00207713-47-2-284
发表时间：
1997-04-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
Avgustin, G;Wallace, RJ;Flint, HJ
通讯作者：
Flint, HJ