Biological performance of cell‐encapsulated methacrylated gellan gum‐based hydrogels for nucleus pulposus regeneration
Biological performance of cell‐encapsulated methacrylated gellan gum‐based hydrogels for nucleus pulposus regeneration
复制标题
细胞封装的甲基丙烯酸结冷胶水凝胶用于髓核再生的生物学性能
DOI：
10.1002/term.1959
复制
发表时间：
2017
影响因子：
3.3
通讯作者：
Kirkpatrick CJ
中科院分区：
文献类型：
--
作者：
Tsaryk R;Silva-Correia J;Oliveira JM;Unger RE;Landes C;Brochhausen C;Ghanaati S;Reis RL;Kirkpatrick CJ
当前治疗椎间盘退变的局限性促使人们对组织工程方法的发展产生了兴趣。装载细胞的可注射水凝胶可作为内IVD部分髓核（NP）的替代材料，为IVD退变的微创治疗提供了机会。NP由软骨细胞样细胞填充；因此，刺激软骨细胞和间充质干细胞（MSCs）沿软骨谱系分化，可用于促进NP再生。在这项研究中，研究了人骨髓来源的间充质干细胞和鼻腔软骨细胞（NCs）对改性结冷胶基水凝胶的体外黄素反应。离子（iGG-MA）和光交联（phGG-MA）甲基丙烯酸结冷胶水凝胶在MSCs和nc的提取实验中均显示没有细胞毒性。此外，这些材料不会诱导内皮细胞的促炎反应。此外，尽管在phGG-MA中观察到凋亡，但MSCs和NCs可以被包裹在水凝胶中并保持至少2周的活性。重要的是，被包膜的MSCs和nc在皮下植入iGG-MA时显示出体内软骨形成的迹象。总之，这些数据支持了改性结冷胶基水凝胶作为NP组织工程中合适材料的潜在用途。版权所有©2014 John Wiley & Sons， Ltd。
Limitations of current treatments for intervertebral disc (IVD) degeneration have promoted interest in the development of tissue‐engineering approaches. Injectable hydrogels loaded with cells can be used as a substitute material for the inner IVD part, the nucleus pulposus (NP), and provide an opportunity for minimally invasive treatment of IVD degeneration. The NP is populated by chondrocyte‐like cells; therefore, chondrocytes and mesenchymal stem cells (MSCs), stimulated to differentiate along the chondrogenic lineage, could be used to promote NP regeneration. In this study, thein vitroandin vivoresponse of human bone marrow‐derived MSCs and nasal chondrocytes (NCs) to modified gellan gum‐based hydrogels was investigated. Both ionic‐ (iGG–MA) and photo‐crosslinked (phGG–MA) methacrylated gellan gum hydrogels show no cytotoxicity in extraction assays with MSCs and NCs. Furthermore, the materials do not induce pro‐inflammatory responses in endothelial cells. Moreover, MSCs and NCs can be encapsulated into the hydrogels and remain viable for at least 2 weeks, although apoptosis is observed in phGG–MA. Importantly, encapsulated MSCs and NCs show signs ofin vivochondrogenesis in a subcutaneous implantation of iGG–MA. Altogether, the data endorse the potential use of modified gellan gum‐based hydrogel as a suitable material in NP tissue engineering. Copyright © 2014 John Wiley & Sons, Ltd.
登录
查看更多内容
金属植入物对炎症和愈合过程的影响
影响因子：
0.8
作者：
R. Tsaryk;K. Peters;R. Unger;D. Scharnweber;C. Kirkpatrick
通讯作者：
C. Kirkpatrick
影响因子：
19.7
作者：
Kurunlahti, M;Kerttula, L;Tervonen, O
通讯作者：
Tervonen, O
影响因子：
10
作者：
Silva-Correia, Joana;Zavan, Barbara;Reis, Rui L.
通讯作者：
Reis, Rui L.
影响因子：
4.5
作者：
Horn, P.;Bork, S.;Wagner, W.
通讯作者：
Wagner, W.
影响因子：
3.3
作者：
Silva-Correia, J.;Oliveira, J. M.;Reis, R. L.
通讯作者：
Reis, R. L.