Analysis of human serum and whole blood for mineral content by ICP-MS and ICP-OES: development of a mineralomics method.
Analysis of human serum and whole blood for mineral content by ICP-MS and ICP-OES: development of a mineralomics method.
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DOI：
10.1007/s12011-014-0033-5
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发表时间：
2014-07
影响因子：
3.9
通讯作者：
Levine, Keith E.
中科院分区：
文献类型：
--
作者：
Harrington, James M.;Young, Daniel J.;Essader, Amal S.;Sumner, Susan J.;Levine, Keith E.
矿物质是无机化合物，对支持各种生物功能至关重要。了解生物样品中这些矿物质含量的范围和变化可以深入了解矿物质含量与个人健康之间的关系。特别是，异常的矿物质含量可以作为疾病的指标。开发用于测定生物样品中矿物质含量的稳健、可靠的分析方法对于开发用于了解矿物质与疾病之间关系的生物模型至关重要。本文描述了一种用于分析健康个体的小体积血清和全血样品的矿物质含量的方法。测定了血液（250 μl）和血清（250 μl）样本中8种必需矿物质（钠（Na）、钙（Ca）、镁（Mg）、钾（K）、铁（Fe）、锌（Zn）、铜（Cu）、血清和全血测定的相对标准偏差（RSD）分别为0.635 - 10.1%和0.348 - 5.98%。比较10份血清样本和6份全血样本的测定范围，结果与文献参考范围一致。结果表明，消化和分析方法可以用于可靠地测量这些矿物质的含量，并可能添加其他矿物质。
Minerals are inorganic compounds that are essential to the support of a variety of biological functions. Understanding the range and variability of the content of these minerals in biological samples can provide insight into the relationships between mineral content and the health of individuals. In particular, abnormal mineral content may serve as an indicator of illness. The development of robust, reliable analytical methods for the determination of the mineral content of biological samples is essential to developing biological models for understanding the relationship between minerals and illnesses. This manuscript describes a method for the analysis of the mineral content of small volumes of serum and whole blood samples from healthy individuals. Interday and intraday precision for the mineral content of the blood (250 μl) and serum (250 μl) samples was measured for eight essential minerals, sodium (Na), calcium (Ca), magnesium (Mg), potassium (K), iron (Fe), zinc (Zn), copper (Cu), and selenium (Se) by plasma spectrometric methods and ranged from 0.635 – 10.1% relative standard deviation (RSD) for serum and 0.348 – 5.98% for whole blood. A comparison of the determined ranges for ten serum samples and six whole blood samples provided good agreement with literature reference ranges. The results demonstrate that the digestion and analysis methods can be used to reliably measure the content of these minerals, and potentially to add other minerals.
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影响因子：
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