Plasma concentration of parasite DNA as a measure of disease severity in falciparum malaria.
Plasma concentration of parasite DNA as a measure of disease severity in falciparum malaria.
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寄生虫DNA的血浆浓度作为恶性疟疾疾病严重程度的量度。
DOI：
10.1093/infdis/jiu590
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发表时间：
2015-04-01
期刊：
影响因子：
--
通讯作者：
White NJ
中科院分区：
文献类型：
--
作者：
Imwong M;Woodrow CJ;Hendriksen IC;Veenemans J;Verhoef H;Faiz MA;Mohanty S;Mishra S;Mtove G;Gesase S;Seni A;Chhaganlal KD;Day NP;Dondorp AM;White NJ
在疟疾流行地区，恶性疟原虫寄生虫血症在表面健康的儿童中很常见，严重疟疾通常在偶然寄生虫血症的患者中被误诊。我们评估了血浆恶性疟原虫DNA浓度是否是区分非洲儿童和亚洲成人中单纯性疟疾和严重疟疾的有用数据。通过实时聚合酶链反应（PCR）测量了224名非洲儿童（111名无并发症疟疾和113名重度疟疾）和211名亚洲成人（100名无并发症疟疾和111名重度疟疾）的恶性疟原虫DNA浓度。血浆恶性疟原虫DNA浓度对儿童和成人重度疟疾的诊断准确率分别为0.834和0.788，与血浆恶性疟原虫HRP 2水平相似，明显上级寄生虫密度（P <0.0001）。血浆恶性疟原虫DNA浓度加血浆恶性疟原虫HRP 2浓度的诊断准确性显著高于单独血浆恶性疟原虫HRP 2浓度的诊断准确性（儿童为0.904 [P = .004]，成人为0.847 [P = .003]）。实时荧光定量PCR检测血浆中的疟原虫DNA是一种有用的方法，用于诊断新鲜或存档血浆样本中的严重恶性疟疾。
In malaria-endemic areas, Plasmodium falciparum parasitemia is common in apparently healthy children and severe malaria is commonly misdiagnosed in patients with incidental parasitemia. We assessed whether the plasma Plasmodium falciparum DNA concentration is a useful datum for distinguishing uncomplicated from severe malaria in African children and Asian adults. P. falciparum DNA concentrations were measured by real-time polymerase chain reaction (PCR) in 224 African children (111 with uncomplicated malaria and 113 with severe malaria) and 211 Asian adults (100 with uncomplicated malaria and 111 with severe malaria) presenting with acute falciparum malaria. The diagnostic accuracy of plasma P. falciparum DNA concentrations in identifying severe malaria was 0.834 for children and 0.788 for adults, similar to that of plasma P. falciparum HRP2 levels and substantially superior to that of parasite densities (P < .0001). The diagnostic accuracy of plasma P. falciparum DNA concentrations plus plasma P. falciparum HRP2 concentrations was significantly greater than that of plasma P. falciparum HRP2 concentrations alone (0.904 for children [P = .004] and 0.847 for adults [P = .003]). Quantitative real-time PCR measurement of parasite DNA in plasma is a useful method for diagnosing severe falciparum malaria on fresh or archived plasma samples.
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DOI：
10.4269/ajtmh.2009.09-0274
发表时间：
2009-11-01
影响因子：
3.3
作者：
Bharti, Ajay R.;Letendre, Scott L.;Smith, Davey M.
通讯作者：
Smith, Davey M.
影响因子：
3
作者：
Ramutton T;Hendriksen IC;Mwanga-Amumpaire J;Mtove G;Olaosebikan R;Tshefu AK;Onyamboko MA;Karema C;Maitland K;Gomes E;Gesase S;Reyburn H;Silamut K;Chotivanich K;Promnares K;Fanello CI;von Seidlein L;Day NP;White NJ;Dondorp AM;Imwong M;Woodrow CJ
通讯作者：
Woodrow CJ
影响因子：
9.4
作者：
Kamau, Edwin;Tolbert, LaDonna S.;Ockenhouse, Christian F.
通讯作者：
Ockenhouse, Christian F.
影响因子：
168.9
作者：
Dondorp, Arjen M.;Fanello, Caterina I.;White, Nicholas J.
通讯作者：
White, Nicholas J.
影响因子：
3.7
作者：
Gamboa D;Ho MF;Bendezu J;Torres K;Chiodini PL;Barnwell JW;Incardona S;Perkins M;Bell D;McCarthy J;Cheng Q
通讯作者：
Cheng Q