Secretome diversity and quantitative analysis of cellulolytic Aspergillus fumigatus Z5 in the presence of different carbon sources.
Secretome diversity and quantitative analysis of cellulolytic Aspergillus fumigatus Z5 in the presence of different carbon sources.
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DOI：
10.1186/1754-6834-6-149
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发表时间：
2013-10-16
影响因子：
6.3
通讯作者：
Shen Q
中科院分区：
文献类型：
--
作者：
Liu D;Li J;Zhao S;Zhang R;Wang M;Miao Y;Shen Y;Shen Q
烟曲霉Z5具有很强的分解木质纤维素生物质的能力，其胞外蛋白分泌在早期采用传统技术的研究中已有报道。然而，仍然缺乏在不同碳源存在下其分泌的综合分析。这项工作的目标是鉴定、量化和比较不同碳源存在下的烟曲霉 Z5 的分泌组，以更详细地了解烟曲霉 Z5 分解木质纤维素的机制。纤维素分解烟曲霉Z5在葡萄糖(Gl)、微晶纤维素(Av)和稻草(RS)存在下生长，并用比色法测定几种木质纤维素酶的活性。内切葡聚糖酶、外切葡聚糖酶、β-葡萄糖苷酶、昆布多糖酶、地衣多糖酶、木聚糖酶和果胶裂解酶的最大活性分别为12.52、0.59、2.30、2.37、1.68、15.02和11.40 U·ml-1。在RS、Av和Gl存在下分别鉴定出总共152、125和61种不同的蛋白质，并且这些蛋白质在功能上分为糖苷水解酶、脂肪酶、肽酶、过氧化物酶、酯酶、蛋白质易位转运蛋白和假设蛋白质。所有处理中总共对49种蛋白质进行了iTRAQ定量，定量结果表明，当稻草和微晶纤维素作为碳源时（与葡萄糖相比），大多数纤维素酶、半纤维素酶和糖苷水解酶均高度上调。通过LC-MS/MS鉴定烟曲霉Z5在不同碳源条件下分泌的蛋白质，并通过基于iTRAQ的定量蛋白质组学进行定量。结果表明，烟曲霉Z5可以产生大量的纤维素、半纤维素、果胶和木质素降解酶，这些酶对于木质纤维素生物能源工业具有重要价值。
Aspergillus fumigatus Z5 has a strong ability to decompose lignocellulose biomass, and its extracellular protein secretion has been reported in earlier studies employing traditional techniques. However, a comprehensive analysis of its secretion in the presence of different carbon sources is still lacking. The goal of this work was to identify, quantify and compare the secretome of A. fumigatus Z5 in the presence of different carbon sources to understand in more details the mechanisms of lignocellulose decomposition by Aspergillus fumigatus Z5. Cellulolytic A. fumigatus Z5 was grown in the presence of glucose (Gl), Avicel (Av) and rice straw (RS), and the activities of several lignocellulosic enzymes were determined with chromatometry method. The maximum activities of endoglucanase, exoglucanase, β-glucosidase, laminarinase, lichenase, xylanase and pectin lyase were 12.52, 0.59, 2.30, 2.37, 1.68, 15.02 and 11.40 U·ml-1, respectively. A total of 152, 125 and 61 different proteins were identified in the presence of RS, Av and Gl, respectively, and the proteins were functionally divided into glycoside hydrolases, lipases, peptidases, peroxidases, esterases, protein translocating transporters and hypothetical proteins. A total of 49 proteins were iTRAQ-quantified in all the treatments, and the quantification results indicated that most of the cellulases, hemicellulases and glycoside hydrolases were highly upregulated when rice straw and Avicel were used as carbon sources (compared with glucose). The proteins secreted from A. fumigatus Z5 in the present of different carbon source conditions were identified by LC-MS/MS and quantified by iTRAQ-based quantitative proteomics. The results indicated that A. fumigatus Z5 could produce considerable cellulose-, hemicellulose-, pectin- and lignin-degrading enzymes that are valuable for the lignocellulosic bioenergy industry.
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DOI：
10.1007/bf01569654
发表时间：
1994-01-01
期刊：
JOURNAL OF INDUSTRIAL MICROBIOLOGY
影响因子：
--
作者：
HOLT, SM;HARTMAN, PA
通讯作者：
HARTMAN, PA
影响因子：
4.4
作者：
Adav, Sunil S.;Ng, Chee Sheng;Sze, Siu Kwan
通讯作者：
Sze, Siu Kwan
影响因子：
3.3
作者：
Adav, Sunil S.;Ng, Chee Sheng;Sze, Siu Kwan
通讯作者：
Sze, Siu Kwan
影响因子：
2.6
作者：
Herculano, Polyanna N.;Porto, Tatiana S.;Porto, Ana L. F.
通讯作者：
Porto, Ana L. F.
DOI：
10.1016/j.ibiod.2011.04.005
发表时间：
2011-08-01
影响因子：
4.8
作者：
Liu, Dongyang;Zhang, Ruifu;Shen, Qirong
通讯作者：
Shen, Qirong