Evolutionary adaptation of the amino acid and codon usage of the mosquito sodium channel following insecticide selection in the field mosquitoes.
Evolutionary adaptation of the amino acid and codon usage of the mosquito sodium channel following insecticide selection in the field mosquitoes.
复制标题
在蚊子中选择杀虫剂后，蚊子通道的氨基酸和密码子使用的进化适应。
DOI：
10.1371/journal.pone.0047609
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Liu N
中科院分区：
文献类型：
--
作者：
Xu Q;Zhang L;Li T;Zhang L;He L;Dong K;Liu N
昆虫神经系统电压门控钠通道内的点突变导致的靶位不敏感性是已知的对拟除虫菊酯类杀虫剂产生抗药性的首要因素。这项研究改变了当前的研究范式，首次对致倦库蚊整个蚊子钠通道中所有自然发生的突变，包括非同义和同义突变，以及突变组合进行了全球分析，并分析了它们的进化和遗传特征及其在抗药性中的作用。通过系统比较敏感品系与抗性品系、田间亲本蚊虫与其氯菊酯精选后代个体之间、不同蚊群对氯氰菊酯耐受性水平的不同蚊种之间的核苷酸多态，鉴定出3个非同义突变(A109S、L982F和W1573R)和6个同义突变(L852、G891、A1241、D1245、P1249和G1733)。所有9个突变的共存，包括非同义和同义突变，以及它们的纯合性被发现是导致高水平抗药性的重要因素。我们的研究首次提供了一个强有力的案例，证明了抗药性蚊子钠通道中非同义和同义突变共存，以及这些突变在选择杀虫剂后的野外蚊子株后代中的遗传。
Target site insensitivity resulting from point mutations within the voltage-gated sodium channel of the insect nervous system is known to be of primary importance in the development of resistance to pyrethroid insecticides. This study shifts current research paradigms by conducting, for the first time, a global analysis of all the naturally occurring mutations, both nonsynonymous and synonymous mutations, as well as mutation combinations in the entire mosquito sodium channel of Culex quinquefasciatus and analyzing their evolutionary and heritable feature and roles in insecticide resistance. Through a systematic analysis of comparing nucleotide polymorphisms in the entire sodium channel cDNAs of individuals between susceptible and resistant mosquito strains, between field parental mosquitoes and their permethrin selected offspring, and among different mosquito groups categorized by their levels of tolerance to specific permethrin concentrations within and among the mosquito strains of the field parental strains and their permethrin selected offspring, 3 nonsynonymous (A109S, L982F, and W1573R) and 6 synonymous (L852, G891, A1241, D1245, P1249, and G1733) mutations were identified. The co-existence of all 9 mutations, both nonsynonymous and synonymous, and their homozygousity were found to be important factors for high levels of resistance. Our study, for the first time, provide a strong case demonstrating the co-existence of both nonsynonymous and synonymous mutations in the sodium channel of resistant mosquitoes in response to insecticide resistance and the inheritance of these mutations in the offspring of field mosquito strains following insecticide selection.
登录
查看更多内容
影响因子：
3.8
作者：
Burton, Mark J.;Mellor, Ian R.;Williamson, Martin S.
通讯作者：
Williamson, Martin S.
影响因子：
1.9
作者：
Brengues, C;Hawkes, NJ;Hemingway, J
通讯作者：
Hemingway, J
DOI：
10.1073/pnas.89.22.10905
发表时间：
1992-11-15
影响因子：
11.1
作者：
PATTON, DE;WEST, JW;GOLDIN, AL
通讯作者：
GOLDIN, AL
影响因子：
2.1
作者：
Chapman, Juliet;Whittaker, John
通讯作者：
Whittaker, John
影响因子：
2.1
作者：
Liu, Nannan;Xu, Qiang;Zhang, Lee
通讯作者：
Zhang, Lee