Imaging transient blood vessel fusion events in zebrafish by correlative volume electron microscopy.
Imaging transient blood vessel fusion events in zebrafish by correlative volume electron microscopy.
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DOI：
10.1371/journal.pone.0007716
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发表时间：
2009-11-06
期刊：
影响因子：
3.7
通讯作者：
Collinson LM
中科院分区：
文献类型：
--
作者：
Armer HE;Mariggi G;Png KM;Genoud C;Monteith AG;Bushby AJ;Gerhardt H;Collinson LM
生物过程的研究越来越依赖于通过成像技术获得高分辨率的空间和时间数据。由于研究人员需要在整个生物体的背景下对细胞事件进行分子分辨率，因此在复杂的三维样品中将非侵入性活生物体成像与电子显微镜相关联变得至关重要。脊椎动物发育中的血管形成了一个高度复杂的网络，使用传统方法无法以高分辨率成像。在这里，我们表明，在活的共聚焦显微镜鉴定的转基因斑马鱼的血管之间的融合点，随后可以通过使用聚焦离子束/扫描电子显微镜（FIB/SEM）和连续块面/扫描电子显微镜（SBF/SEM）的有机体的结构进行跟踪。由此产生的数据提供了前所未有的显微解剖学细节的斑马鱼，并首次允许可视化的超微结构的时间有限的生物事件的背景下，一个完整的有机体。
The study of biological processes has become increasingly reliant on obtaining high-resolution spatial and temporal data through imaging techniques. As researchers demand molecular resolution of cellular events in the context of whole organisms, correlation of non-invasive live-organism imaging with electron microscopy in complex three-dimensional samples becomes critical. The developing blood vessels of vertebrates form a highly complex network which cannot be imaged at high resolution using traditional methods. Here we show that the point of fusion between growing blood vessels of transgenic zebrafish, identified in live confocal microscopy, can subsequently be traced through the structure of the organism using Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) and Serial Block Face/Scanning Electron Microscopy (SBF/SEM). The resulting data give unprecedented microanatomical detail of the zebrafish and, for the first time, allow visualization of the ultrastructure of a time-limited biological event within the context of a whole organism.
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