A comparative study of Pointed and Yan expression reveals new complexity to the transcriptional networks downstream of receptor tyrosine kinase signaling.
A comparative study of Pointed and Yan expression reveals new complexity to the transcriptional networks downstream of receptor tyrosine kinase signaling.
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DOI：
10.1016/j.ydbio.2013.11.002
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发表时间：
2014-01-15
影响因子：
2.7
通讯作者：
Carthew, Richard W.
中科院分区：
文献类型：
--
作者：
Lachance, Jean-Francois Boisclair;Pelaez, Nicolas;Cassidy, Justin J.;Webber, Jemma L.;Rebay, Ilaria;Carthew, Richard W.
受体酪氨酸激酶（RTK）信号传导下游的生化调节网络由两个相对的ETS家族成员控制：转录激活因子Pointed（Pnt）和转录抑制因子Yan。已经调用了一个转换开关模型来解释通路激活如何通过将系统从高Yan/低Pnt活性状态转换为低Yan/高Pnt活性状态来驱动分化。尽管该模型解释了几种不同细胞类型中的Yan和Pnt功能丧失表型，但Yan和Pointed蛋白表达动力学如何促进这些和其他发育转变仍然知之甚少。为了实现这一目标，我们使用了一个功能性的GFP标记的Pnt转基因（Pnt-GFP）进行比较研究的Yan和Pnt蛋白表达在整个果蝇发育。与Pnt-Yan网络的流行模型一致，我们发现了许多Pnt-GFP和Yan采用相互排斥的表达模式的例子。然而，我们也观察到了许多共表达的例子。虽然一些共表达发生在假定RTK信号传导低的细胞中，但其他共表达发生在具有高RTK信号传导的细胞中。在具有高RTK信号传导的组织中共表达的Yan和Pnt-GFP的实例不能由当前模型解释，因此它们为将来研究RTK信号传导、网络拓扑结构或对其他信号传导输入的响应性中的上下文特异性差异如何影响转录响应提供了重要背景。
The biochemical regulatory network downstream of receptor tyrosine kinase (RTK) signaling is controlled by two opposing ETS family members: the transcriptional activator Pointed (Pnt) and the transcriptional repressor Yan. A bistable switch model has been invoked to explain how pathway activation can drive differentiation by shifting the system from a high-Yan/low-Pnt activity state to a low-Yan/high-Pnt activity state. Although the model explains yan and pnt loss-of-function phenotypes in several different cell types, how Yan and Pointed protein expression dynamics contribute to these and other developmental transitions remains poorly understood. Toward this goal we have used a functional GFP-tagged Pnt transgene (Pnt-GFP) to perform a comparative study of Yan and Pnt protein expression throughout Drosophila development. Consistent with the prevailing model of the Pnt-Yan network, we found numerous instances where Pnt-GFP and Yan adopt a mutually exclusive pattern of expression. However we also observed many examples of co-expression. While some co-expression occurred in cells where RTK signaling is presumed low, other co-expression occurred in cells with high RTK signaling. The instances of co-expressed Yan and Pnt-GFP in tissues with high RTK signaling cannot be explained by the current model, and thus they provide important contexts for future investigation of how context-specific differences in RTK signaling, network topology, or responsiveness to other signaling inputs, affect the transcriptional response.
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影响因子：
64.8
作者：
BRUNNER, D;DUCKER, K;KLAMBT, C
通讯作者：
KLAMBT, C
影响因子：
10.5
作者：
HART, AC;KRAMER, H;ZIPURSKY, SL
通讯作者：
ZIPURSKY, SL
DOI：
10.1007/bf00848157
发表时间：
1984-01-01
期刊：
WILHELM ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY
影响因子：
--
作者：
JURGENS, G;WIESCHAUS, E;KLUDING, H
通讯作者：
KLUDING, H
影响因子：
4.6
作者：
Caviglia, Sara;Luschnig, Stefan
通讯作者：
Luschnig, Stefan
影响因子：
4.6
作者：
Cinnamon, Einat;Helman, Aharon;Paroush, Zeev
通讯作者：
Paroush, Zeev