Towards the cell-instructive bactericidal substrate: exploring the combination of nanotopographical features and integrin selective synthetic ligands.
Towards the cell-instructive bactericidal substrate: exploring the combination of nanotopographical features and integrin selective synthetic ligands.
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DOI：
10.1038/s41598-017-16385-3
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发表时间：
2017-11-27
影响因子：
4.6
通讯作者：
Mas-Moruno C
中科院分区：
文献类型：
--
作者：
Fraioli R;Tsimbouri PM;Fisher LE;Nobbs AH;Su B;Neubauer S;Rechenmacher F;Kessler H;Ginebra MP;Dalby MJ;Manero JM;Mas-Moruno C
设计生物材料和组织之间的界面对于延长植入物的寿命、避免失败和翻修手术非常重要。永久性设备应加强干细胞的附着和分化，负责损伤组织的修复，同时阻止细菌定植；这是一个重大挑战。为了向这种多功能表面迈出第一步，我们建议在临床相关材料(如钛)的表面上融合地形和生化线索。具体来说，我们的策略结合了抗菌纳米拓扑学特征和整合素选择性合成配体，可以挽救表面的黏附能力，并指示间充质干细胞(MSC)的反应。为此，已经制备了光滑的衬底和两种不同的高深宽比拓扑图，并涂覆了αvβ3选择性多肽、α5β1选择性多肽或RGd/PHSRN肽分子。结果表明，该底物对致病性铜绿假单胞菌的抑菌效果较好。此外，功能化增加了间充质干细胞与表面的粘附性，αvβ3选择性模拟肽纳米拓扑图促进了成骨。这种实现多功能表面的双重物理化学方法是设计新型细胞指导型生物材料表面的第一步。
Engineering the interface between biomaterials and tissues is important to increase implant lifetime and avoid failures and revision surgeries. Permanent devices should enhance attachment and differentiation of stem cells, responsible for injured tissue repair, and simultaneously discourage bacterial colonization; this represents a major challenge. To take first steps towards such a multifunctional surface we propose merging topographical and biochemical cues on the surface of a clinically relevant material such as titanium. In detail, our strategy combines antibacterial nanotopographical features with integrin selective synthetic ligands that can rescue the adhesive capacity of the surfaces and instruct mesenchymal stem cell (MSC) response. To this end, a smooth substrate and two different high aspect ratio topographies have been produced and coated either with an αvβ3-selective peptidomimetic, an α5β1-selective peptidomimetic, or an RGD/PHSRN peptidic molecule. Results showed that antibacterial effects of the substrates could be maintained when tested on pathogenic Pseudomonas aeruginosa. Further, functionalization increased MSC adhesion to the surfaces and the αvβ3-selective peptidomimetic-coated nanotopographies promoted osteogenesis. Such a dual physicochemical approach to achieve multifunctional surfaces represents a first step in the design of novel cell-instructive biomaterial surfaces.
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影响因子：
3.2
作者：
Auernheimer, J;Zukowski, D;Kessler, H
通讯作者：
Kessler, H
影响因子：
4.6
作者：
Diu T;Faruqui N;Sjöström T;Lamarre B;Jenkinson HF;Su B;Ryadnov MG
通讯作者：
Ryadnov MG
影响因子：
5
作者：
Hasan, Jafar;Webb, Hayden K.;Ivanova, Elena P.
通讯作者：
Ivanova, Elena P.
DOI：
10.1073/pnas.0903269107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Kilian, Kristopher A.;Bugarija, Branimir;Mrksich, Milan
通讯作者：
Mrksich, Milan
影响因子：
16.6
作者：
Ivanova, Elena P.;Hasan, Jafar;Webb, Hayden K.;Gervinskas, Gediminas;Juodkazis, Saulius;Vi Khanh Truong;Wu, Alex H. F.;Lamb, Robert N.;Baulin, Vladimir A.;Watson, Gregory S.;Watson, Jolanta A.;Mainwaring, David E.;Crawford, Russell J.
通讯作者：
Crawford, Russell J.