Evaluation of delivery conditions for cutaneous plasmid electrotransfer using a multielectrode array.
Evaluation of delivery conditions for cutaneous plasmid electrotransfer using a multielectrode array.
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电穿孔(EP)是一种简单的体内方法，可以将正常情况下不渗透的分子，如质粒DNA，输送到各种组织中。由于电极对之间的距离以及施加的电压必须增加才能有效地渗透细胞膜，所以用EP将质粒DNA输送到较大的表面积是不现实的。多电极阵列(MEA)的设计结合了固定距离的多个电极对，以允许将质粒DNA输送到皮肤，潜在地减少了与体内电穿孔相关的感觉。在这份报告中，我们使用编码荧光素酶报告基因的质粒，通过皮内注射在豚鼠模型中评估了场强和脉冲宽度对转基因表达和持续时间的影响。正如预期的那样，荧光素酶的表达水平随着施加电压的大小和持续时间而增加。除了通过改变电场强度来调节转基因表达水平外，还可以通过调节质粒剂量来控制转基因表达水平。我们的结果表明，MEA的设计是一种可行的选择，通过体内EP将质粒DNA输送到较大的表面积。
Electroporation (EP) is a simple in vivo method to deliver normally impermeable molecules, such as plasmid DNA, to a variety of tissues. Delivery of plasmid DNA by EP to a large surface area is not practical because the distance between the electrode pairs, and therefore the applied voltage, must be increased to effectively permeabilize the cell membrane. The design of the MultiElectrode Array (MEA) incorporates multiple electrode pairs at a fixed distance to allow for delivery of plasmid DNA to the skin potentially reducing the sensation associated with in vivo electroporation. In this report, we evaluate the effects of field strength and pulse width on transgene expression and duration using a plasmid encoding the luciferase reporter gene delivered by intradermal injection in a guinea pig model followed by EP with the MEA. As expected, the level of luciferase expression increased with the magnitude and duration of the voltage applied. In addition to adjusting transgene expression levels by altering fielding strength, levels could also be controlled by adjusting the plasmid dose. Our results indicate that the design of the MEA is a viable option for cutaneous plasmid DNA delivery by in vivo EP to a large surface area.
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影响因子：
4.2
作者：
Heller, Richard;Cruz, Yolmari;Jaroszeski, Mark J.
通讯作者：
Jaroszeski, Mark J.
影响因子：
5.1
作者：
Heller, L;Jaroszeski, MJ;Heller, R
通讯作者：
Heller, R
影响因子：
5.1
作者：
Gothelf, A.;Hojman, P.;Gehl, J.
通讯作者：
Gehl, J.
影响因子：
5.1
作者：
Heller, L. C.;Jaroszeski, M. J.;Heller, R.
通讯作者：
Heller, R.
影响因子：
12.4
作者：
Glasspool-Malone, J;Somiari, S;Malone, RW
通讯作者：
Malone, RW