A constitutive damage-specific DNA-binding protein is synthesized at higher levels in UV-irradiated primate cells
A constitutive damage-specific DNA-binding protein is synthesized at higher levels in UV-irradiated primate cells
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在紫外线照射的灵长类动物细胞中合成了更高水平的组成型损伤特异性 DNA 结合蛋白
DOI：
10.1128/mcb.10.5.2041-2048.1990
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发表时间：
1990
影响因子：
5.3
通讯作者：
M. Protic
中科院分区：
文献类型：
--
作者：
S. Hirschfeld;A. Levine;K. Ozato;M. Protic
利用DNA带移分析，我们在灵长类细胞中鉴定出一种DNA结合蛋白复合体，它是由组成存在的，与紫外线照射的双链DNA具有很高的亲和力。经紫外光、丝裂霉素C或aphidicolin处理的细胞有更高水平的这种损伤特异性DNA结合蛋白复合体，这表明诱导信号可能是对DNA的损伤，也可能是对细胞DNA复制的干扰。DNA的物理化学修饰和与特定底物的竞争分析表明，损伤特异性DNA结合蛋白复合体最有可能的靶点是6-4‘-(嘧啶-2’-酮)-嘧啶二聚体：含有-TT-环丁烷二聚体的探针不能检测到特异性结合，紫外线照射的DNA光激活后，损伤特异性DNA结合没有减少。这种损伤特异的DNA结合蛋白复合体是在灵长类细胞中发现的第一个这样的可诱导蛋白复合体。来自阳光敏感型癌症易感疾病--着色性干皮病患者(E组)的细胞缺乏结构性和诱导性损伤特异性DNA结合活性。这些发现表明，这种DNA结合蛋白复合体可能在紫外线诱导的光产物的损伤识别和DNA修复中发挥作用。
Using a DNA band shift assay, we have identified a DNA-binding protein complex in primate cells which is present constitutively and has a high affinity for UV-irradiated, double-stranded DNA. Cells pretreated with UV light, mitomycin C, or aphidicolin have higher levels of this damage-specific DNA-binding protein complex, suggesting that the signal for induction can either be damage to the DNA or interference with cellular DNA replication. Physiochemical modifications of the DNA and competition analysis with defined substrates suggest that the most probable target site for the damage-specific DNA-binding protein complex is a 6-4'-(pyrimidine-2'-one)-pyrimidine dimer: specific binding could not be detected with probes which contain -TT- cyclobutane dimers, and damage-specific DNA binding did not decrease after photoreactivation of UV-irradiated DNA. This damage-specific DNA-binding protein complex is the first such inducible protein complex identified in primate cells. Cells from patients with the sun-sensitive cancer-prone disease, xeroderma pigmentosum (group E), are lacking both the constitutive and the induced damage-specific DNA-binding activities. These findings suggest a possible role for this DNA-binding protein complex in lesion recognition and DNA repair of UV-light-induced photoproducts.
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影响因子：
56.9
作者：
CHU, G;CHANG, E
通讯作者：
CHANG, E
DOI：
10.1073/pnas.78.6.3388
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LIPPKE, JA;GORDON, LK;HASELTINE, WA
通讯作者：
HASELTINE, WA
影响因子：
16.6
作者：
G. C. Walker
通讯作者：
G. C. Walker
DOI：
--
发表时间：
1984-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Sancar;F. W. Smith;G. Sancar
通讯作者：
A. Sancar;F. W. Smith;G. Sancar
人类细胞损伤特异性 DNA 结合蛋白的底物特异性。
影响因子：
14.9
作者：
Feldberg,RS
通讯作者：
Feldberg,RS