N-glycans of the porcine nematode parasite Ascaris suum are modified with phosphorylcholine and core fucose residues.
N-glycans of the porcine nematode parasite Ascaris suum are modified with phosphorylcholine and core fucose residues.
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DOI：
10.1111/j.1742-4658.2006.05615.x
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发表时间：
2007-02
期刊：
影响因子：
--
通讯作者：
Wilson IB
中科院分区：
文献类型：
--
作者：
Pöltl G;Kerner D;Paschinger K;Wilson IB
近年来，许多寄生线虫的糖缀合物由于其免疫原性和免疫调节性而引起了人们的兴趣。先前对猪蛔虫寄生虫蛔虫的研究主要集中在其鞘糖脂上，发现其部分被磷酸胆碱修饰。使用质谱法和Western blotting，我们现在分析了该物种成虫的PNGase a释放的n -聚糖。通过LC/ESI-Q-TOF-MS/MS验证了杂化、双链和三链n -聚糖的存在，其中一些是由核心α1,6-焦点和外周磷胆碱修饰的，还有一些是由核心α1,3-焦点修饰的少甘露聚糖和低甘露聚糖修饰的。Western blotting证实了蛋白结合的磷酸胆碱和核心α1,3- focus的存在，而糖基转移酶检测显示存在核心α1,6- focusyltransferase和lewis型α1,3- focusyltransferase的活性。虽然在模型线虫秀丽隐杆线虫中没有发现不寻常的三聚和四聚聚糖，但在a . suum中发现的绝大多数n -聚糖代表了秀丽隐杆线虫中发现的n -聚糖的一个子集；因此，我们的数据表明，后者是寄生线虫的一个有趣的糖生物学模型。
In recent years, the glycoconjugates of many parasitic nematodes have attracted interest due to their immunogenic and immunomodulatory nature. Previous studies with the porcine roundworm parasite Ascaris suum have focussed on its glycosphingolipids which were found, in part, to be modified by phosphorylcholine. Using mass spectrometry and Western blotting, we have now analysed the PNGase A-released N-glycans of adults of this species. The presence of hybrid, bi- and triantennary N-glycans, some modified by core α1,6-fucose and peripheral phosphorylcholine, was demonstrated by LC/ESI-Q-TOF-MS/MS, as was the presence of paucimannosidic N-glycans, some of which carry core α1,3-fucose, and oligomannosidic oligosaccharides. Western blotting verified the presence of protein-bound phosphorylcholine and core α1,3-fucose, whereas glycosyltransferase assays showed the presence of core α1,6-fucosyltransferase and Lewis-type α1,3-fucosyltransferase activities. Although, the unusual tri- and tetrafucosylated glycans found in the model nematode Caenorhabditis elegans were not found, the vast majority of the N-glycans found in A. suum represent a subset of those found in C. elegans; thus, our data demonstrate that the latter is an interesting glycobiological model for parasitic nematodes.
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