Targeting cancer cell-specific RNA interference by siRNA delivery using a complex carrier of affibody-displaying bio-nanocapsules and liposomes.
Targeting cancer cell-specific RNA interference by siRNA delivery using a complex carrier of affibody-displaying bio-nanocapsules and liposomes.
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DOI：
10.1186/1477-3155-11-19
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发表时间：
2013-06-24
影响因子：
10.2
通讯作者：
Kondo A
中科院分区：
文献类型：
--
作者：
Nishimura Y;Mieda H;Ishii J;Ogino C;Fujiwara T;Kondo A
小干扰RNA（siRNA）作为RNA干扰（RNAi）应用在核酸医学领域引起了关注，其由于特异性信使RNA（mRNA）破坏而导致基因沉默。然而，由于siRNA在血液中不稳定并且不能穿过细胞膜，因此需要将siRNA包封到载体中。在这项研究中，我们使用了一种载体，结合ZHER 2展示生物纳米胶囊（来自B病毒表面抗原）和脂质体的复合物，以调查有效的和靶细胞特异性的RNAi应用的可行性。因此，通过仅在表达HER 2的乳腺癌细胞中观察RNAi，使用我们提出的方法，我们成功地证明了siRNA的靶细胞特异性递送和有效功能表达。这些发现表明，在核酸药物领域，ZHER 2-BNC/LP可以成为siRNA递送的有用载体，也可以成为基因沉默和实现蛋白质敲除的有用工具。
Small interfering RNA (siRNA) has attracted attention in the field of nucleic acid medicine as a RNA interference (RNAi) application that leads to gene silencing due to specific messenger RNA (mRNA) destruction. However, since siRNA is unstable in blood and unable to cross the cell membrane, encapsulation of siRNA into a carrier is required. In this study, we used a carrier that combined ZHER2-displaying bio-nanocapsule (derived from hepatitis B virus surface antigen) and liposomes in a complex in order to investigate the feasibility of effective and target-cell-specific RNAi applications. As a result, by observing RNAi only in HER2-expressing breast cancer cells, using our proposed methodology, we successfully demonstrated target-cell-specific delivery and effective function expression of siRNA. These findings show that, in the field of nucleic acid medicine, ZHER2-BNC/LP can be a useful carrier for siRNA delivery, and could also become a useful tool for gene silencing and to accomplish protein knock-down.
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