Differentiation of otitis media-causing bacteria and biofilms via Raman spectroscopy and optical coherence tomography.
Differentiation of otitis media-causing bacteria and biofilms via Raman spectroscopy and optical coherence tomography.
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DOI：
10.3389/fcimb.2022.869761
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发表时间：
2022
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
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作者：
在中耳炎（OM）的治疗中，鉴定致病细菌病原体和了解其生物膜的形成可以提供更有针对性的治疗方法。目前的临床诊断方法依赖于鼓膜的可视化，缺乏对致病病原体的实时评估，以及可能存在于鼓膜后和中耳腔内的任何生物膜的性质。近年来，光学相干断层扫描（OCT）已被证明是一种改进的体内诊断工具，用于OM生物膜和中耳积液的可视化和形态学表征；但对致病细菌种类缺乏特异性。本研究提出结合OCT和拉曼光谱（RS）来检测流感嗜血杆菌、肺炎链球菌、卡他莫拉菌和铜绿假单胞菌的折射率、光衰减和生化组成的差异；OM的四种主要耳病原体。这种组合为在三种不同的体外生长环境（即琼脂培养菌落、液体培养的浮游细胞和生物膜）下识别和区分引起om的细菌种类提供了双重光学方法。本研究表明，RS能够识别关键的生化变异，以区分所有四种引起om的细菌。此外，生化光谱变化（RS）和平均衰减系数（OCT）的差异可以区分每种细菌的生长环境。
In the management of otitis media (OM), identification of causative bacterial pathogens and knowledge of their biofilm formation can provide more targeted treatment approaches. Current clinical diagnostic methods rely on the visualization of the tympanic membrane and lack real-time assessment of the causative pathogen(s) and the nature of any biofilm that may reside behind the membrane and within the middle ear cavity. In recent years, optical coherence tomography (OCT) has been demonstrated as an improved in vivo diagnostic tool for visualization and morphological characterization of OM biofilms and middle ear effusions; but lacks specificity about the causative bacterial species. This study proposes the combination of OCT and Raman spectroscopy (RS) to examine differences in the refractive index, optical attenuation, and biochemical composition of Haemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis, and Pseudomonas aeruginosa; four of the leading otopathogens in OM. This combination provides a dual optical approach for identifying and differentiating OM-causing bacterial species under three different in vitro growth environments (i.e., agar-grown colonies, planktonic cells from liquid cultures, and biofilms). This study showed that RS was able to identify key biochemical variations to differentiate all four OM-causing bacteria. Additionally, biochemical spectral changes (RS) and differences in the mean attenuation coefficient (OCT) were able to distinguish the growth environment for each bacterial species.
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影响因子：
3.6
作者：
Allesen-Holm, M;Barken, KB;Tolker-Nielsen, T
通讯作者：
Tolker-Nielsen, T
DOI：
10.1016/j.saa.2004.11.017
发表时间：
2005-05-01
影响因子：
4.4
作者：
Krafft, C;Neudert, L;Salzer, R
通讯作者：
Salzer, R
影响因子：
--
作者：
Kaya, Ercan;Dag, Ilknur;Birdane, Leman
通讯作者：
Birdane, Leman
影响因子：
2.2
作者：
Bakke, R;Kommedal, R;Kalvenes, S
通讯作者：
Kalvenes, S
DOI：
10.1039/c7ay00128b
发表时间：
2017-03-28
期刊：
Analytical methods : advancing methods and applications
影响因子：
--
作者：
Ayala OD;Wakeman CA;Pence IJ;O'Brien CM;Werkhaven JA;Skaar EP;Mahadevan-Jansen A
通讯作者：
Mahadevan-Jansen A